Genome-wide reannotation of the transcriptional and functional landscape of Acinetobacter baumannii ATCC 17978

ABSTRACT: Acinetobacter baumannii is an important opportunistic pathogen, but its regulatory architectures and protein functions remain elucidated. Here, we systematically characterized regulatory elements and protein functions in A. baumannii ATCC 17978. By integrating genome analysis, RNA-Seq data, and bioinformatic annotation, we identified 91 previously unrecognized small RNAs, 3334 transcription start sites, 649 operons, and multiple strong endogenous promoters, substantially refining the regulatory landscape of this organism. The validity and function of representative regulatory elements were confirmed by qRT-PCR, 5’-RACE, and GFP reporter assays. In parallel, sequence- and structure-based annotation increased the functional coverage of hypothetical proteins in ATCC 17978 to 82.9%, with growth inhibition assays demonstrating the toxicity of four candidates annotated as toxins. We further integrated these data with existing resources into an open-access AB database. Together, these results expand the regulatory and functional repertoire of A. baumannii and provide insight into studying gene regulation and antimicrobial target discovery.

ORGANISM(S): Acinetobacter baumannii

PROVIDER: GSE342665 | GEO | 2026/10/01

REPOSITORIES: GEO