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Salmonella Typhimurium: Wild type SL1344 Vs. ∆relA, ∆spoT (ppGpp0) strain grown under SPI1-inducing conditions

ABSTRACT: Transcriptional profiling of early stationary phase S. Typhimurium, comparing wild type SL1344 with an isogenic ∆relA,∆spoT strain (JH3370).

ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91 Salmonella enterica subsp. enterica serovar Typhimurium str. DT104 Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 Salmonella enterica subsp. enterica serovar Enteritidis

PROVIDER: GSE34269 | GEO | 2011/12/09

SECONDARY ACCESSION(S): PRJNA149961

REPOSITORIES: GEO

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Salmonella Typhimurium: Wild type SL1344 Vs. ∆relA, ∆spoT (ppGpp0) strain grown under SPI1-inducing conditions

Project description:Transcriptional profiling of early stationary phase S. Typhimurium, comparing wild type SL1344 with an isogenic ∆relA,∆spoT strain (JH3370). Each array used labelled cDNA against a common genomic DNA reference. The wild type strain was arrayed in triplicate and the ∆relA,∆spoT strain in quadruplicate using RNA isolated from biologically independent cultures.

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Project description:WT and mutant strains (relA-, relA-spoT-) were submitted to starvation for isoleucine. 40 minutes after starvation, RNA was extracted. All samples were compared to RNA from rapidly growing WT cells in identical medium.<br><br> A data file with additional processed data is available for download from <a href="ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MEXP/E-MEXP-1370/">ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MEXP/E-MEXP-1370/</a>

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Salmonella Typhimurium SL1344 RiboPools/RiboZero comparison

Project description:Comparison of RiboPools and RiboZero rRNA depletion strategies in total RNA from Salmonella Typhimurium, strain SL1344

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Project description:We performed transcriptome abundance analysis of Salmonella Typhimurium strain SL1344 swap which has been genetically engineered to express the hns open-reading frame from the stpA promoter and the stpA open reading frame from the hns promoter. This strain is designated SL1344(swap). Transcript abundance was compared with that of wild-type SL1344. This comparison was performed to determine the effect of chromosome location of the expression of two related global regulators and how alterations to their expression patterns would impact on their regulons.

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Expression analysis of Salmonella enterica serovar Typhimurium strain SL1344 with and without plasmid R27

Project description:Investigation of gene expression level changes in Salmonella Typhiumurium SL1344 (R27) compared to the wild-type SL1344 strain when grown at different growth temperatures and growth phases.

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Project description:Transcriptional profiling of Salmonella enterica serovar Typhimurium SL1344 cells comparing wildtype with ΔfabR mutant. Salmonella strains were cultured under free-living TSB conditions (TSB 1/20, 200 rpm, 25 °C) for 6h (ca. 2 x 108 cells/ml).

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