Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Whole transcriptome sequencing reveals dynamic RNA changes in the aging rat brain

ABSTRACT: Using RNA-seq, 9 cerebral cortex RNA samples were sequenced from 6 month (n=3, 6 individuals pooled), 12 month (n=3, 6 individuals pooled) and 28 month (n=3, 6 individuals pooled) rats. Allowing brain aging in the cerebral cortex to be assessed.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE34272 | GEO | 2012/05/10

SECONDARY ACCESSION(S): PRJNA149963

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Whole transcriptome sequencing reveals dynamic RNA changes in the aging rat brain

Project description:Using RNA-seq, 9 cerebral cortex RNA samples were sequenced from 6 month (n=3, 6 individuals pooled), 12 month (n=3, 6 individuals pooled) and 28 month (n=3, 6 individuals pooled) rats. Allowing brain aging in the cerebral cortex to be assessed. Transcriptional profiling of the ageing cerebral cortex at 6, 12 and 28 months

2012-05-09 | E-GEOD-34272 | biostudies-arrayexpress

Transcriptome analysis in calorie-restricted rats implicates epigenetic and post-translational mechanisms in neuroprotection and aging

Project description:Using RNA-seq, 39 cerebral cortex RNA samples were sequenced. The study design was as follows: Ad libitum fed rats at 6 months (n=3, 6 individuals pooled), 12 months (n=3, 6 individuals pooled) and 28 months (n=3, 6 individuals pooled). Calorie restricted rats at 6 months (n=3, 6 individuals pooled), 12 months (n=3, 6 individuals pooled) and 28 months (n=3, 6 individuals pooled). Rats fed alpha lipoic acid as a supplement to ad libitum at 12 months (n=3, 6 individuals pooled) and 28 months (n=3, 6 individuals pooled). Diet switching groups, where diet was changes at 12 months; 28 month ad libitum switched to calorie restriction (n=3, 6 individuals pooled), 28 month calorie restriction switched to ad libitum (n=3, 6 individuals pooled), 28 month ad libitum plus lipoic acid switched to calorie restriction (n=3, 6 individuals pooled), 28 month calorie restriction switched to ad libitum plus lipoic acid (n=3, 6 individuals pooled).

2015-11-22 | GSE57110 | GEO

Transcriptome analysis in calorie-restricted rats implicates epigenetic and post-translational mechanisms in neuroprotection and aging

2015-11-22 | E-GEOD-57110 | biostudies-arrayexpress

Profiling of spatio-temporally regulated transcripts during cerebral corticogenesis in the mouse

Project description:The cerebral cortex plays an important role in cognitive function and specialized perception in mammals and its development requires highly specific spatio-temporal control of gene expression. The study identified stage- and region-specific markers throughout cerebral corticogenesis at various important stages of cerebral cortex development; embryonic day (E) 15.5, E17.5, postnatal day (P) 1.5 and 4-6 months old. The study involved the analysis of 12 SAGE libraries, which were generated from the mouse cerebral cortex of E15.5 (n=3), E17.5 (n=2), P1.5 (n=1) and 4-6 month old (n=6). N denotes biological replicates.

2009-09-25 | E-GEOD-15031 | biostudies-arrayexpress

Expression of known and predicted genes in tissues of Gallus gallus (chicken)

Project description:Expression of known and predicted genes in tissues of Gallus gallus (chicken) pooled from multiple healthy individuals. Two-colour experiments with two different tissues hybridized to each array. Each tissue is arrayed in replicate with dye swaps. Tissues: Bursa of Fabricius, Cerebellum, Cerebral cortex, Eye, Femur with bone marrow, Gallbladder, Gizzard, Heart, Intestine, Kidney, Liver, Lung, Muscle, Ovary, Oviduct, Skin, Spleen, Stomach, Testis, Thymus

2010-06-19 | E-GEOD-12974 | biostudies-arrayexpress

The TMT-based quantitative proteomics of 5×FAD_Usp25 knockout mouse

Project description:The TMT-based quantitative proteomic raw data of 6-month-old WT, 5×FAD, 5×FAD:Usp25 KO mouse cerebral cortex including hippcampus.

2020-09-22 | PXD021637 | iProX

Widespread splicing changes in human brain development and aging

Project description:Differences in transcript splicing are well documented among tissues and between sexes in humans and other organisms. Further, splicing changes in selected genes have been reported in human and mouse brain development. Using high-throughput RNA sequencing we characterized the transcriptome-wide splicing changes occurring over the lifespan in the human brain. We found that in two brain regions, prefrontal cortex and cerebellum, as many as 37% of all expressed protein-coding multi-exon genes undergo significant splicing changes between birth and 98 years of age. Approximately 40% of these changes take place in aging. We confirmed our findings using exon arrays and PCRs and detected identified splicing changes at the protein level. We further associated distinct patterns of age-related splicing changes with expression of the corresponding splicing factors. Thus, splicing plays an important role in shaping the human brain transcriptome in both development and aging. Human post-mortem brain samples from prefrontal cortex and cerebellum from 30 individuals with ages from birth to 100 years were collected. For each five individuals of similar age RNA, extracted from dissected tissue, was pooled resulting in 5 pooled samples. Obtained RNA was hybridized with Affymetrix Human Exon 1.0 ST arrays.

2013-01-22 | E-GEOD-26586 | biostudies-arrayexpress

Time-Dependent Effects of Anesthetic Administration on the Rat Hypothalamus and Cerebral Cortex Peptidome

Project description:Prohormone-derived neuropeptides act as cell-cell signaling molecules to mediate a wide variety of biological processes in the animal brain. Mass spectrometry-based peptidomic experiments are valuable approaches to gain insight into the dynamics of individual peptides under different physiological conditions or experimental treatments. However, the use of anesthetics during animal procedures may confound experimental peptide measurements, especially in the brain where anesthetics act. Here, we investigate the effect of the commonly used anesthetics isoflurane and sodium pentobarbital on the peptide profile in the rodent hypothalamus and cerebral cortex, as assessed by label-free quantitative peptidomics. Our results showed that neither anesthetic dramatically alters peptide levels, although extended isoflurane exposure did cause changes in a small number prohormone-derived peptides in the cerebral cortex. Overall, our results demonstrate that acute use of anesthetics can be used in peptidomic experiments of the hypothalamus and cerebral cortex without dramatic confounding of experimental results.

2023-10-25 | PXD043738 | Pride

DNA methylation in neurons from post-mortem brains in schizophrenia and bipolar disorder (RNA-Seq)

Project description:We fine-mapped DNA methylation in neuronal nuclei (NeuN+) isolated by flow cytometry from post-mortem frontal cortex of the brain of individuals diagnosed with schizophrenia, bipolar disorder, and controls (n=29, 26, and 28 individuals).

2019-05-03 | GSE112523 | GEO