Dataset Information


Murine ES Cells: Control vs. Triple-Fusion Transfected

ABSTRACT: Transcriptional profiling of mouse embryonic stem cells comparing control untreated ES cells with ES cells transfected with a pUb-fluc-mrfp-ttk triple fusion reporter gene. The latter makes ES visualization possible by FACS and single cell microscopy, high throughput bioluminescence imaging, and PET. Goal was to determine the effects of triple-fusion (TF) gene transfection on global ES gene expression. Keywords: Genetic modification Overall design: Two-condition experiment, ES vs. TF-ES cells. Biological replicates: 4 control, 3 transfected, independently grown and harvested. One replicate per array.

INSTRUMENT(S): Agilent Mouse Development Oligo Microarray (G4120A) Reannotated 020504

SUBMITTER: Joshua M. Spin  

PROVIDER: GSE3432 | GEO | 2005-10-12



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Transcriptional profiling of reporter genes used for molecular imaging of embryonic stem cell transplantation.

Wu Joseph C JC   Spin Joshua M JM   Cao Feng F   Lin Shuan S   Xie Xiaoyan X   Gheysens Olivier O   Chen Ian Y IY   Sheikh Ahmad Y AY   Robbins Robert C RC   Tsalenko Anya A   Gambhir Sanjiv S SS   Quertermous Tom T  

Physiological genomics 20060103 1

Stem cell therapy offers exciting promise for treatment of ischemic heart disease. Recent advances in molecular imaging techniques now allow investigators to monitor cell fate noninvasively and repetitively. Here we examine the effects of a triple-fusion reporter gene on embryonic stem (ES) cell transcriptional profiles. Murine ES cells were stably transfected with a self-inactivating lentiviral vector carrying a triple-fusion (TF) construct consisting of fluorescence, bioluminescence, and posit  ...[more]

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