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Analysis of 3' 2'-O-methylated small RNAs in Caenorhabditis elegans

ABSTRACT: To identify methylated small RNAs in C. elegans, we deep sequenced both β-eliminated and untreated small RNAs isolated from wild type C. elegans.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE34321 | GEO | 2011/12/10

SECONDARY ACCESSION(S): PRJNA156417

REPOSITORIES: GEO

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Analysis of 3' 2'-O-methylated small RNAs in Caenorhabditis elegans

Project description:To identify methylated small RNAs in C. elegans, we deep sequenced both β-eliminated and untreated small RNAs isolated from wild type C. elegans. Small RNAs were isolated from larval and adult stage C. elegans and either subjected to β-elimination or no treatment. Small RNA cDNA libraries were sequenced on an Illumina HiSeq instrument, and enrichment or depletion of small RNAs by β-elimination was assessed after library size normalization based on the number of mappable reads in each library.

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