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Uremic Serum Alters Gene Expression Profiles and Signaling Pathway Activity

ABSTRACT: Uremic conditions are common in end-stage kidney disease (ESKD) patients. Accelerated vascular diseases in uremic patients lead to heart failure, stroke, and hypertension. To investigate the effects of uremia on porcine arterial and venous smooth muscle cells (aSMCs and vSMCs), bulk RNA sequencing was used to identify uremia-induced alterations in signaling pathways of aSMCs that might explain the aggressive cardiovascular diseases seen in patients with chronic kidney disease (CKD) and ESKD. Bulk RNA sequencing was performed on porcine aSMCs cultured with serum from normal or uremic pigs. Differentially expressed genes (DEGs) analysis revealed that 295 genes were upregulated, and 138 genes were downregulated in aSMCs after uremic serum exposure. In vSMCs, DEGs analysis revealed that 408 genes were upregulated, and 387 genes were downregulated after uremic serum treatment.

ORGANISM(S): Sus scrofa domesticus

PROVIDER: GSE343584 | GEO | 2026/08/17

REPOSITORIES: GEO

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