A two-hit mechanism triggers autoimmune hair loss

ABSTRACT: Autoimmune diseases arise from a breakdown of immune tolerance, yet the mechanisms that initiate tissue-specific autoimmunity remain poorly understood. Here, we identify a two-hit mechanism in which immune dysregulation and physiological neural activation converge to trigger autoimmune alopecia. We show that transient depletion of regulatory T cells (Tregs) during the anagen phase of the hair cycle, but not during telogen, induces CD8+ T cell-mediated destruction of immune-privileged hair follicle stem cells (HFSCs), resulting in hair loss. This anagen-restricted susceptibility depends on sympathetic nerve activity, which is physiologically elevated during anagen: sympathetic denervation prevents disease, whereas psychological stress or optogenetic activation of sympathetic nerves renders otherwise resistant telogen skin susceptible to autoimmune attack following Treg depletion. Mechanistically, sympathetic norepinephrine signaling through HFSC-intrinsic ADRB2 promotes physiological reactivation of endogenous retroviruses (ERVs) in anagen HFSCs. Under conditions of Treg depletion, this ERV-associated state drives autoimmune pathology by engaging AIM2-dependent innate immune activation and pathogenic CD8+ T cell responses. Accordingly, genetic ablation of Aim2 or pharmacological inhibition of ERV reverse transcription protects against disease development. Together, these findings show that physiological sympathetic activity establishes a transiently vulnerable epithelial state, whereas Tregs prevent this state from progressing to tissue-specific autoimmunity. This neuro–epithelial–immune circuit links hair-cycle state to the breach of HFSC immune privilege and the initiation of autoimmune alopecia.

ORGANISM(S): Mus musculus

PROVIDER: GSE343613 | GEO | 2026/08/18

REPOSITORIES: GEO