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Pseudomonas putida KT2440 : Control vs. recG gene mutant with untreated or paraquat (PQ) treated

ABSTRACT: Transcriptional profiling of P. putida KT2440 cells comparing control untreated wild type cells with untreated recG gene mutant cells or PQ treated recG gene mutant cells

ORGANISM(S): Pseudomonas putida Pseudomonas putida KT2440

PROVIDER: GSE34410 | GEO | 2011/12/15

SECONDARY ACCESSION(S): PRJNA149651

REPOSITORIES: GEO

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Pseudomonas putida KT2440 : Control vs. recG gene mutant with untreated or paraquat (PQ) treated

Project description:Transcriptional profiling of P. putida KT2440 cells comparing control untreated wild type cells with untreated recG gene mutant cells or PQ treated recG gene mutant cells Three-condition experiment, Control vs. untreated recG gene mutant and PQ treated recG gene mutant. Biological replicates: 4 samples independently grown and harvested

2011-12-15 | E-GEOD-34410 | biostudies-arrayexpress

Pseudomonas putida KT2440 : Control vs. paraquat (PQ) or cumen hydroperoxide (CHP) treated

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Pseudomonas putida KT2440 : Control vs. paraquat (PQ) or cumen hydroperoxide (CHP) treated

Project description:Transcriptional profiling of P. putida KT2440 cells comparing control untreated cells with PQ or CHP treated cells Three-condition experiment, Control vs. treatment. Biological replicates: 1 control, 3 treated, independently grown and harvested

2011-12-15 | E-GEOD-34409 | biostudies-arrayexpress

Pseudomonas putida KT2440

Project description:Pseudomonas putida KT2440 : Control vs. recG gene mutant with untreated or paraquat (PQ) treated

| PRJNA149651 | ENA

Pseudomonas putida KT2440: Control vs. indole 1mM treated

Project description:Transcriptional profiling of P. putida KT2440 cells comparing control untreated cells with 1 mM indole treated cells

2012-10-02 | GSE41254 | GEO

Pseudomonas putida KT2440 : Control vs. indole or ampicillin or indole plus ampicillin treated

Project description:Transcriptional profiling of P. putida KT2440 cells comparing untreated cells with 1 mM indole or 50 μg/ml ampicillin or 1 mM indole plus 50 μg/ml ampicillin treated cells

2016-09-10 | GSE86617 | GEO

Pseudomonas putida KT2440 : Control vs. indole 1mM treated

Project description:Transcriptional profiling of P. putida KT2440 cells comparing control untreated cells with 1 mM indole treated cells Two-condition experiment, Control vs. treatment. Biological replicates: 2 control, 2 treated, independently grown and harvested

2012-10-02 | E-GEOD-41254 | biostudies-arrayexpress

The effect of multiple phaJ deletions Pseudomonas putida KT2440 on polyhydroxyalkanoates synthesis

Project description:Pseudomonas putida KT2440 is a well-known model organism for the medium chain length (mcl) PHA accumulation. (R)-Specific enoyl-coenzyme A hydratase (PhaJ) was considered to be the main supplier of monomers for PHA synthesis by converting the -oxidation intermediate, trans-2-enoyl-CoA to (R)-3-hydroxyacyl-CoA when fatty acids (FA) are used. Three PhaJ homologues, PhaJ1, PhaJ4 and MaoC are annotated in P. putida KT2440. To investigate the relationship of fatty acids - PHA metabolism and the role of each PhaJ in PHA biosynthesis in P. putida KT2440, a series of P. putida KT2440 knockouts was obtained. PHA content and monomer composition in WT and mutants under different growth conditions were analysed. However, when all three PhaJ homologues were deleted, the mutant still accumulated PHA up to 10.7 % of the cell dry weight (CDW). To identify other potential PHA monomer suppliers by analysing the proteome of the phaJ1maoCphaJ4. The proteomes of the WT, phaJ1phaJ4 and phaJ1maoCphaJ4 strains in MSM medium with octanoate under nitrogen limited condition were detected. In addition, we found that the deletion of PhaJ1 in P. putida KT2440 has a negative impact on the PHA accumulation in cells cultivated on glucose with nitrogen limitation conditions. It seems PhaJ1 also mediates the synthesis of PHA when glucose was used as the carbon and energy source. To investigate the role of PhaJ1 in PHA accumulation with glucose, the proteomes of P. putida KT2440 wild type, phaJ1, phaJ1phaJ4 and phaJ1maoCphaJ4 mutant growing on glucose were detected and compared.

2023-05-10 | PXD037932 | Pride

Pseudomonas putida KT2440

Project description:Pseudomonas putida KT2440 : Control vs. paraquat (PQ) or cumen hydroperoxide (CHP) treated

| PRJNA149649 | ENA

Expression data from Pseudomonas putida KT2440 Tn5 mutants

Project description:We used Progenika oligonucleotide arrays to monitor the gene expression after cold shock from 30°C to 10°C. The 10°C samples of the P. putida wild type were compared to those of the respective P. putida KT2440 Tn5 mutants affected in either cbrA (PP4695), cbrB (PP4696), pcnB (PP4697), vacB (PP4880) or bipA (PP5044).

2014-02-01 | GSE28257 | GEO

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