Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from transfection of U87 glioma cells with miR-376a* for 72 hours

ABSTRACT: Several biological pathways can be under the regulation of miRNAs. These pathways can be indentified by the enforced expression of a miRNA and analysing the expression data for enrichment of specific pathways represented among the genes differentially expressed upon miRNA overexpression. We transfected unedited miR-376a* (376a*A) and edited miR-376a* (376a*G) into U87 cells to identify differentially regulated transcripts. List of differentially expressed genes were subject to pathway enrichment analysis to identify specific biological processes regulated by each of miR-376a*A and miR-376a*G.

ORGANISM(S): Homo sapiens

PROVIDER: GSE34456 | GEO | 2012/06/01

SECONDARY ACCESSION(S): PRJNA156353

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Expression data from transfection of U87 glioma cells with miR-376a* for 72 hours

Project description:Several biological pathways can be under the regulation of miRNAs. These pathways can be indentified by the enforced expression of a miRNA and analysing the expression data for enrichment of specific pathways represented among the genes differentially expressed upon miRNA overexpression. We transfected unedited miR-376a* (376a*A) and edited miR-376a* (376a*G) into U87 cells to identify differentially regulated transcripts. List of differentially expressed genes were subject to pathway enrichment analysis to identify specific biological processes regulated by each of miR-376a*A and miR-376a*G. U87 cells (100,000) were transfected with 50 nM of miR-376a*A, miR-376a*G or miR-control. Following incubation for 72 hours, total RNA was isolated from transfected cells. For each (miR-376a*A or miR-376a*G), genes differentially expressed upon miRNA transfection were determined based on comparison with miR-control-transfected samples. Biological triplicates were used for each sample type.

2012-05-31 | E-GEOD-34456 | biostudies-arrayexpress

Expression data from transfection of U87 glioma cells with miR-376a* for 24 hours

Project description:Enforced expression of miRNAs in cells leads to down-regulation of several mRNAs, which harbour binding sites in their 3'UTRs for the overexpressed miRNA and represent potential target genes of the miRNA We transfected unedited miR-376a* (376a*A) and edited miR-376a* (376a*G) into U87 cells to identify transcripts down-regulated by each in comparison to control miRNA transfection. The aim was to identify target genes directly subject to regulation by miR-376a*A and by miR-376a*G U87 cells (100,000) were transfected with 50 nM of miR-376a*A, miR-376a*G or miR-control. Following incubation for 24 hours, total RNA was isolated from transfected cells. For each (miR-376a*A or miR-376a*G), genes differentially expressed upon miRNA transfection were determined based on comparison with miR-control-transfected samples. Biological triplicates were used for each sample type.

2012-05-31 | E-GEOD-34455 | biostudies-arrayexpress

Expression data from transfection of U87 glioma cells with miR-376a* for 24 hours

2012-06-01 | GSE34455 | GEO

Expression data from transfection of SW1783 glioma cells with microRNA-376a* for 24 hours

Project description:Enforced expression of miRNAs in cells leads to down-regulation of several mRNAs which harbour binding sites in their 3'UTRs for the overexpressed miRNA and represent potential target genes of the miRNA We transfected unedited miR-376a* (376a*A) and edited miR-376a* (376a*G) into SW1783 cells to identify transcripts down-regulated by each in comparison to control miRNA transfection. The aim was to identify target genes subject to direct regulation by miR-376a*A and by miR-376a*G. SW1783 cells (100,000) were transfected with 50 nM of miR-376a*A, miR-376a*G or miR-control. Following incubation for 24 hours, total RNA was isolated from transfected cells. For each (miR-376a*A or miR-376a*G), genes differentially expressed upon miRNA transfection were determined based on comparison with miR-control-transfected samples. Biological triplicates were used for each sample type.

2012-05-31 | E-GEOD-34454 | biostudies-arrayexpress

Expression data from transfection of SW1783 glioma cells with microRNA-376a* for 24 hours

2012-06-01 | GSE34454 | GEO

Expression data from transfection of SW1783 and U87 glioma cells with miR-376a* for 24 and 72 hours

Project description:This SuperSeries is composed of the following subset Series: GSE34454: Expression data from transfection of SW1783 glioma cells with microRNA-376a* for 24 hours GSE34455: Expression data from transfection of U87 glioma cells with miR-376a* for 24 hours GSE34456: Expression data from transfection of U87 glioma cells with miR-376a* for 72 hours Refer to individual Series

2012-05-31 | E-GEOD-34482 | biostudies-arrayexpress

miRNA expression profile in leprosy skin biopsy specimens

Project description:Skin biopsy specimens of skin lesions were profiled for miRNA expression. In this study, we indentified miRNA species that were differentially expressed in the skin lesions of either the lepromatous or tuberculoid forms of leprosy. One miRNA species, hsa-mir-21, found in the lepromatous lesions was capable of downregulating the vitamin D-dependent antimicrobial pathway.

2011-12-31 | GSE33192 | GEO

Differential microRNA editing may drive target pathway switching in human temporal lobe epilepsy

Project description:Transcriptional profiling of human-iPSC derived neurons treated with antimir-edited-miR-376a-3p at site +6 (Anta-E), antimir-native-miR-376a-3p (Anta-N) and scramble (Scr)

2024-01-03 | GSE211696 | GEO

QK-knockdown U87 transduced with miR-20a or a scrambled non-targeting microRNA (miR-NT)

Project description:Identify potential miR-20a regulated mRNAs and linked pathways in the setting of QK knockdown by comparing the transcriptional profiles of shQK-transduced human U87 cells together with miR-20a or a scrambled miRNA control (miR-NT)

2009-12-30 | GSE19690 | GEO

Integrative analysis of mRNA-miRNA expression profiles and somatic variants in oxysterol signaling in early-stage luminal breast cancer

Project description:Oxysterols, oxidized derivatives of cholesterol, act in breast cancer (BC) as selective estrogen receptor modulators and affect cholesterol homeostasis, drug transport, nuclear and cell receptors, and other signaling proteins. Using overlapping data from patients with early-stage estrogen receptor-positive BC—high-coverage targeted DNA sequencing (99 patients, 113 genes), mRNA sequencing (67 patients), and full miRNome by microarrays (123 patients)—we describe complex mRNA-miRNA and miRNA-miRNA interaction (correlation) networks, with validation in two carefully curated public datasets (n=538 in total) and 11 databases. The ESR1-CH25H-INSIG1-ABCA9 axis was the most prominent, being interconnected through hsa-miR-125b-5p, but also hsa-miR-99a-5p, hsa-miR-100-5p, hsa miR 143 3p, hsa-199b-5p, hsa-miR-376a-3p, and hsa-miR-376c-3p. Mutations in SC5D, CYP46A1, and its functionally linked gene set were associated with multiple differentially expressed genes. STARD5 was upregulated in patients with positive lymph node status. High expression of miR-19b-3p was weakly associated with poor survival in multiple datasets. This is the first detailed dedicated study of interactions between DNA variation and mRNA expression of oxysterol-related genes, the miRNA transcriptome, and clinical factors in BC.

2023-08-02 | GSE225327 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data