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CD84 Determines Differential Fates of Pro-fibrotic Macrophages [scRNA-seq]

ABSTRACT: Fibrotic interstitial lung diseases (fILDs) are characterized by macrophage heterogeneity and the expansion of SPP1hi pro-fibrotic macrophages. We performed single-cell RNA sequencing (scRNA-seq) of whole-lung tissues from bleomycin-treated control mice and mice with monocyte-specific Cd84 deletion to investigate how CD84 regulates myeloid cell recruitment, differentiation, and function during pulmonary fibrosis. scRNA-seq identified distinct monocyte and macrophage populations. CD84 was preferentially expressed in Spp1hi pro-fibrotic macrophages, and its expression progressively increased along the monocyte-to-pro-fibrotic macrophage differentiation trajectory. Cd84 deletion reduced the recruitment of monocyte-derived macrophages, inhibited their differentiation into Spp1hi macrophages, and shifted macrophage composition toward Il1rnhi macrophages associated with alveolar epithelial regeneration. These findings identify CD84 as a key regulator of macrophage fate and a potential therapeutic target for pulmonary fibrosis.

ORGANISM(S): Mus musculus

PROVIDER: GSE344929 | GEO | 2026/09/25

REPOSITORIES: GEO

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Project description:RATIONALE: Interstitial lung diseases (ILDs) encompass a diverse range of fibrotic conditions and contribute to significant respiratory morbidity and mortality. Assessment of the cellular composition of bronchoalveolar lavage (BAL) fluid is an important diagnostic test in people presenting with ILD, but BAL cellularity remains relatively uncharacterized at single-cell resolution. OBJECTIVE: To characterize immune cell populations in BAL across different ILDs and investigate the impact of shortened peripheral blood leukocyte telomere length on BAL immune profiles. METHODS: Single-cell RNA sequencing and downstream analysis were performed on BAL samples from 25 patients with various ILDs, including idiopathic pulmonary fibrosis (IPF), hypersensitivity pneumonitis, sarcoidosis, and silicosis. Both normal-telomere and short-telomere patients were included. Additionally, we integrated our findings with IPF genome-wide association study (GWAS) data. RESULTS: We identified sixteen distinct cell populations in BAL with notable differences across ILD subtypes. Analysis revealed six monocyte-like macrophage (MLM) subclusters following divergent trajectories: inflammatory CXCL10hi MLMs predominated in hypersensitivity pneumonitis, while pro-fibrotic SPP1hi MLMs were significantly expanded in IPF. Short-telomere patients demonstrated an increased proportion of pro-fibrotic SPP1hi MLMs with enhanced expression of fibrotic genes compared to patients with normal telomere length. Integration with genomic data confirmed that SPP1hi and CCL2hi MLM subclusters harbour cells with the highest IPF disease relevance scores. CONCLUSION: BAL-derived transcriptomics reveals distinct myeloid subpopulations across ILD subtypes, with specific populations associated with disease pathogenesis. These findings provide insight into ILD pathogenesis, motivate the development of more sophisticated diagnostic tests using BAL sampling, and highlight specific myeloid subpopulations as potential therapeutic targets.

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CD84 Determines Differential Fates of Pro-fibrotic Macrophages [scRNA-seq]

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