SRSF1 promotes myogenic differentiation of bovine skeletal muscle satellite cells through post-transcriptional regulation associated with FGF16

ABSTRACT: The objective of this study was to investigate the role of serine/arginine-rich splicing factor 1 (SRSF1) in the myogenic differentiation of bovine skeletal muscle satellite cells and its regulatory relationship with fibroblast growth factor 16 (FGF16). Bovine skeletal muscle satellite cells were subjected to SRSF1 knockdown and overexpression to evaluate their proliferation and differentiation capacities. RNA sequencing and subsequent validation experiments were performed to identify potential downstream targets of SRSF1, while functional assays were conducted to determine the role of FGF16 in myogenesis. RNA immunoprecipitation (RIP) and actinomycin D treatment were further used to investigate RNA-level interactions and transcript stability, and changes in PI3K/AKT signaling activity were assessed by western blotting. The results showed that SRSF1 expression dynamically increased during myogenic differentiation and significantly promoted myotube formation without markedly affecting cell proliferation. Transcriptomic analysis and validation identified FGF16 as a potential downstream target of SRSF1, and functional analyses demonstrated that FGF16 promoted myogenic differentiation. RIP assays suggested an association between SRSF1 mRNA and FGF16-associated complexes, while modulation of SRSF1 expression altered FGF16 expression and the phosphorylation status of PI3K and AKT. Collectively, these findings suggest that SRSF1 may promote the myogenic differentiation of bovine skeletal muscle satellite cells through post-transcriptional regulation associated with FGF16 and modulation of PI3K/AKT signaling activity, providing new insights into RNA-binding protein-mediated regulatory mechanisms involved in bovine skeletal muscle development.

ORGANISM(S): Bos taurus

PROVIDER: GSE344950 | GEO | 2026/08/30

REPOSITORIES: GEO