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Study on screening methylation molecular markers in umbilical cord blood DNA of preterm infants with preeclampsia using Targeted Methylation Sequencing

ABSTRACT: using |Δβ| > 0.1 and adj.P < 0.05 as screening criteria, 23 candidate CpG sites were identified through Targeted Methylation Sequencing (30 cases in the observation group and 30 cases in the control group).

ORGANISM(S): Homo sapiens

PROVIDER: GSE345720 | GEO | 2026/09/03

REPOSITORIES: GEO

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Project description:Understanding the process of adaptation remains a key challenge for strength coaches to enhance athletic performance in basketball players. While physical improvements are commonly used to track progress, these adaptations are underpinned by tightly regulated molecular changes within skeletal muscle. Among these, DNA methylation is an epigenetic mechanism that modulates gene expression in response to training and may play a pivotal role in driving adaptations. This study investigated genome-wide DNA methylation changes following two distinct 30-min, high-intensity basketball-specific interventions, Superset (SS) and Repeated Sprint Training (RST), in youth basketball players. Sixty-six male basketball players (U-13 to U-18) were randomized into SS (N=32) or RST (N=30) groups. Both completed 30-minute weekly sessions for 8 weeks alongside regular basketball training. Saliva samples collected pre- and post-intervention were analysed using Illumina 900K (EPIC v2) microarray. Differentially methylated positions (DMPs) were identified using linear modelling (FDR < 0.05). Biological relevance was further assessed using a Δβ threshold of 0.1. Gene ontology and pathway enrichment analysis (Metascape) was conducted to identify biological pathways potentially modified by the intervention. SS and RST resulted in 112 and 308 DMPs (76 and 73% hypomethylation), respectively, alongside performance improvements. Methylation changes did not reach biological significance threshold (Δβ<0.1). Enrichment analysis for SS identified mTOR, PI3K-Akt and cAMP signalling pathways, whereas enrichment for RST identified VEGF, IGF receptor, MAPK, PI3K-Akt, NF-κB and TGF-β signalling pathways While the magnitude of methylation change (Δβ) was below the threshold commonly considered biologically significant, the presence of DMPs, alongside performance improvements, demonstrated epigenetic changes after high-intensity, 8-week interventions (SS or RST), with enrichment analysis indicating distinct molecular pathways engaged by each training type.

Dataset Information

Study on screening methylation molecular markers in umbilical cord blood DNA of preterm infants with preeclampsia using Targeted Methylation Sequencing

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