Transcriptomic response of apple cv. Ametyst to virulent and avirulent Venturia inaequalis identifies Rin-induced defense candidates

ABSTRACT: Rvi6, introgressed from Malus floribunda 821, is the most widely deployed scab resistance gene in apple, yet Venturia inaequalis isolates able to overcome it are now widespread. How an Rvi6-effective interaction differs transcriptomically from an Rvi6-breaking interaction in the same host genotype has remained unresolved. Own-rooted plants of cv. Ametyst, which carries Rvi6, were inoculated with an avirulent isolate (Rin, from 'Rubin'), a virulent isolate (Rola, from 'Rubinola'), or a distilled-water mock, and leaves were harvested 4 days post-inoculation, giving 21 mRNA libraries. Expression was quantified with two complementary strategies, GDDH13 reference-based and de novo Trinity, followed by differential expression analysis with DESeq2, weighted gene co-expression network analysis and gene-set enrichment analysis. The Rin-versus-Rola contrast yielded 1,339 thresholded-Wald candidates in the reference-based layer; curation of defense-annotated Rin-induced candidates gave 151 genes, 92 of which concentrated in a single co-expression module. The incompatible interaction was associated with a coherent defense program, whereas the compatible interaction showed a distinct transcriptional state.

ORGANISM(S): Malus domestica

PROVIDER: GSE345841 | GEO | 2026/09/02

REPOSITORIES: GEO