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Microarray analysis of polysomal RNA from 5 hours tunicamycin treated U2OS or U2OS-Bmal1 cells

ABSTRACT: Microarray analysis of polysomal RNA from U2OS cells (osteosarcoma) overexprssing Bmal1 or control treated with tunicamycin or vehicle (ER stress inducer) for 5 hours

ORGANISM(S): Homo sapiens

PROVIDER: GSE346128 | GEO | 2026/09/08

REPOSITORIES: GEO

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Microarray analysis of polysomal RNA from 5 hours tunicamycin treated U2OS or U2OS-Bmal1 cells

Project description:Microarray analysis of polysomal RNA from 5 hours tunicamycin treated U2OS or U2OS-Bmal1 cells

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ChIP-seq of three core components of the circadian clock (BMAL1, CLOCK and CRY1) in unsynchronized human U2OS cells

Project description:The human osteosarcoma cell line U2OS contains a functional circadian clock but expresses only a few rhythmic genes. We identified by ChIP-seq analysis 3040 binding sites of the circadian transcription factors BMAL1, CLOCK, and CRY1 in the genome of U2OS cells, comparable to the number found in highly rhythmic tissues like liver. ChIP was performed as described previously (Rey et al, 2011; doi:10.1371/journal.pbio.1000595) using confluent desynchronized U2OS cells and BMAL1, CLOCK, and CRY1-antibodies. Immunoprecipitated chromatin (10 ng DNA of 8 independent BMAL1 ChIPs with enrichment levels > 80-fold, 9 ng DNA of a CRY1 ChIP with 10-fold enrichment, and 8 ng DNA of a CLOCK ChIP with 40-fold enrichment) was used for library preparation. Three lanes of the BMAL1 library, and one lane each of the CRY1 and CLOCK library were sequenced on the Illumina Genome Analyzer IIx using Single-Read Cluster Generation Kit and 36 Cycle Sequencing Kit v2 (Lausanne Genomics Technologies Facility).

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