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MiRNA expression profile in murine un-diseased arterial tissue on 3 month high fat diet vs 10 month high fat diet

ABSTRACT: Aberrantly expressed miRNAs contribute to developmental abnormalities and diseases such as cancer, diabetes, and cardiovascular disorders, by hybridizing to specific mRNA targets and repressing their translation into proteins. Although miRNA expression signature is characterized in the process of neointimal thickening during proliferative vascular diseases such as atherosclerosis, so far global miRNA expression profiling in the different stages of atherosclerosis is completely unknown. We explored stage-specific microRNA signatures in the progress of atherosclerosis in hyperlipidemia mouse model, which may help to identify the critical miRNAs contributing atherosclerotic development and stabilization.

ORGANISM(S): Mus musculus

PROVIDER: GSE34646 | GEO | 2013/03/27

SECONDARY ACCESSION(S): PRJNA156191

REPOSITORIES: GEO

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MiRNA expression profile in murine un-diseased arterial tissue on 3 month high fat diet vs 10 month high fat diet

Project description:Aberrantly expressed miRNAs contribute to developmental abnormalities and diseases such as cancer, diabetes, and cardiovascular disorders, by hybridizing to specific mRNA targets and repressing their translation into proteins. Although miRNA expression signature is characterized in the process of neointimal thickening during proliferative vascular diseases such as atherosclerosis, so far global miRNA expression profiling in the different stages of atherosclerosis is completely unknown. We explored stage-specific microRNA signatures in the progress of atherosclerosis in hyperlipidemia mouse model, which may help to identify the critical miRNAs contributing atherosclerotic development and stabilization. Female apoe-/- mice (6-8 weeks, Jackson Laboratory) were fed a high fat diet (HFD, 21% crude fat, 0.15% cholesterol and 19.5% casein, Altromin, Germany) for 3 or 10 months (N=3-4). Serial sections (20 µm thick) of aortic roots and carotid arteries from these mice were mounted on membrane mounted metal frame slides (MMI), deparaffinized under RNase-free conditions, and completely dried. Laser capture microdissection (LCM) was performed with a laser microdissection system (MMI cellcut plus, Molecular Machines and Industries, Switzerland) assembled onto an inverted microscope (Olympus IX71). Plaque tissue or morphologically normal vessel wall of at least 40 sections per mouse were collected. RNA was isolated using RecoverAll total nucleic acid isolation kit (Applied Biosystems) according to the manufacturer’s instructions.

2013-03-27 | E-GEOD-34646 | biostudies-arrayexpress

MiRNA expression profile in murine early atherosclerotic plaque compared with un-diseased arterial tissue

2013-03-27 | GSE34645 | GEO

MiRNA expression profile in murine advanced atherosclerotic plaque compared with un-diseased arterial wall

2013-03-27 | GSE34644 | GEO

MiRNA expression profile in murine advanced atherosclerotic plaque compared with un-diseased arterial wall

2013-03-27 | E-GEOD-34644 | biostudies-arrayexpress

MiRNA expression profile in murine early atherosclerotic plaque compared with un-diseased arterial tissue

2013-03-27 | E-GEOD-34645 | biostudies-arrayexpress

MiRNA expression profile in murine advanced atherosclerotic plaque compared with early atherosclerotic plaque

2013-03-27 | GSE34647 | GEO

MicroRNA expression profiles in different tissues from normolipidemic hamster and high-fat diet induced hyperlipidemic hamster (Mesocricetus auratus)

Project description:Hyperlipidemia is one of high risk factors for cardiovascular disease (CVD), whose regulation is known to be mediated also by microRNA-mediated mechanisms. Therefore, the analysis of transcriptomics data would gain insights into the molecular mechanisms implicating microRNAs (miRNAs) behind the atherogenic hyperlipidemia. The goal of our study was to profile the specific miRNAs expression associated with atherogenic hyperlipidemia. To this aim, we performed miRNAs microarray analysis for different tissues (liver, left ventricle of the heart, small intestine and blood mononuclear cells) obtained from the experimental model of high-fat diet induced hyperlipidemic hamsters (Mesocricetus auratus). The microarray data were obtained using miRCURY LNA microRNA arrays 7 th generation platform with miRBase 19 (Exiqon, Denmark), using total RNA isolated from hyperlipidemic (HL) and normolipidemic (NL) hamsters? tissues and blood cells. The animals were fed for 12 weeks either standard rodent chow supplemented with 3% cholesterol and 15% unsalted butter (HL) or standard rodent chow (NL), with free access to food and water.

2020-11-26 | GSE128226 | GEO

Differential microRNA response to a high-cholesterol, high-fat diet in livers of low and high LDL-C baboons

2012-07-14 | GSE37249 | GEO

Differential microRNA response to a high-cholesterol, high-fat diet in livers of low and high LDL-C baboons

Project description:Dysregulation of microRNAs (miRNAs) expression has been implicated in molecular genetics events leading to the progression and development of atherosclerosis. We hypothesized that miRNA expression profiles differ between baboons with low and high serum low-density lipoprotein cholesterol (LDL-C) concentrations in response to diet, and that a subset of these miRNAs regulate genes relevant to dyslipidemia and risk of atherosclerosis. We generated small RNA libraries from baboons differing in their LDL-C response to dietary fat and cholesterol (low LDL-C, n = 3; high LDL-C, n = 3) using liver biopsies collected before and after a high-cholesterol, high-fat (HCHF) challenge diet. We sequenced the libraries using Next-Generation Illumina sequencing methods, analyzed the data using mirTools software and identified 517 baboon miRNAs: 490 homologous to human and 27 novel miRNAs. HCHF diet elicited expression of more miRNAs compared to baseline (chow) diet for both low and high LDL-C baboons. Seventeen miRNAs exhibited significant differential expression in response to HCHF diet in high LDL-C baboons compared to nine miRNAs in low LDL-C baboons. Putative miRNA targets were identified with TargetScan/Base tools. miRNAs significantly targeted more genes in high LDL-C baboons compared to low LDL-C responders. Further, we identified miRNA isomers and other non-coding RNAs that were differentially expressed in response to the challenge diet.Our discovery of differentially expressed baboon miRNAs and their targets is a fundamental step in understanding the role of non-coding RNAs in the modulation of dsylipidemia.

2012-07-14 | E-GEOD-37249 | biostudies-arrayexpress

MiRNA expression profile in murine advanced atherosclerotic plaque compared with early atherosclerotic plaque

2013-03-27 | E-GEOD-34647 | biostudies-arrayexpress

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