Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MiRNA expression profile in murine advanced atherosclerotic plaque compared with early atherosclerotic plaque

ABSTRACT: Aberrantly expressed miRNAs contribute to developmental abnormalities and diseases such as cancer, diabetes, and cardiovascular disorders, by hybridizing to specific mRNA targets and repressing their translation into proteins. Although miRNA expression signature is characterized in the process of neointimal thickening during proliferative vascular diseases such as atherosclerosis, so far global miRNA expression profiling in the different stages of atherosclerosis is completely unknown. We explored stage-specific microRNA signatures in the progress of atherosclerosis in hyperlipidemia mouse model, which may help to identify the critical miRNAs contributing atherosclerotic development and stabilization.

ORGANISM(S): Mus musculus

PROVIDER: GSE34647 | GEO | 2013/03/27

SECONDARY ACCESSION(S): PRJNA156365

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

MiRNA expression profile in murine early atherosclerotic plaque compared with un-diseased arterial tissue

2013-03-27 | GSE34645 | GEO

MiRNA expression profile in murine advanced atherosclerotic plaque compared with un-diseased arterial wall

2013-03-27 | GSE34644 | GEO

MiRNA expression profile in murine advanced atherosclerotic plaque compared with early atherosclerotic plaque

Project description:Aberrantly expressed miRNAs contribute to developmental abnormalities and diseases such as cancer, diabetes, and cardiovascular disorders, by hybridizing to specific mRNA targets and repressing their translation into proteins. Although miRNA expression signature is characterized in the process of neointimal thickening during proliferative vascular diseases such as atherosclerosis, so far global miRNA expression profiling in the different stages of atherosclerosis is completely unknown. We explored stage-specific microRNA signatures in the progress of atherosclerosis in hyperlipidemia mouse model, which may help to identify the critical miRNAs contributing atherosclerotic development and stabilization. Female apoe-/- mice (6-8 weeks, Jackson Laboratory) were fed a high fat diet (HFD, 21% crude fat, 0.15% cholesterol and 19.5% casein, Altromin, Germany) for 3 or 10 months (N=3-4). Serial sections (20 µm thick) of aortic roots and carotid arteries from these mice were mounted on membrane mounted metal frame slides (MMI), deparaffinized under RNase-free conditions, and completely dried. Laser capture microdissection (LCM) was performed with a laser microdissection system (MMI cellcut plus, Molecular Machines and Industries, Switzerland) assembled onto an inverted microscope (Olympus IX71). Plaque tissue or morphologically normal vessel wall of at least 40 sections per mouse were collected. RNA was isolated using RecoverAll total nucleic acid isolation kit (Applied Biosystems) according to the manufacturer’s instructions.

2013-03-27 | E-GEOD-34647 | biostudies-arrayexpress

MiRNA expression profile in murine advanced atherosclerotic plaque compared with un-diseased arterial wall

2013-03-27 | E-GEOD-34644 | biostudies-arrayexpress

MiRNA expression profile in murine early atherosclerotic plaque compared with un-diseased arterial tissue

2013-03-27 | E-GEOD-34645 | biostudies-arrayexpress

MiRNA expression profile in murine un-diseased arterial tissue on 3 month high fat diet vs 10 month high fat diet

2013-03-27 | GSE34646 | GEO

MiRNA expression profile in murine un-diseased arterial tissue on 3 month high fat diet vs 10 month high fat diet

2013-03-27 | E-GEOD-34646 | biostudies-arrayexpress

profiles of microRNA expression of atherosclerotic plaques

Project description:To investigate the expression profiles of miRNA in atherosclerotic plaques, the global features of miRNAs expression of three normal coronary artery tissues sample pools and three sample pools of advanced atherosclerosis plaques of coronary artery were studied using microarray technology,

2015-02-20 | E-MTAB-2460 | biostudies-arrayexpress

Unstable Atherosclerotic Plaque Proteome

Project description:The rupture of unstable atherosclerotic plaques, leading to debilitating or fatal thrombotic events, is a major health burden worldwide. Limited understanding as to the molecular drivers of plaque instability and rupture hinders efforts in diagnosis and treatment prior to thrombotic events. Utilising an advanced pre-clinical mouse model (Tandem stenosis (TS) model), which presents human-like unstable atherosclerotic disease, we apply high-end omic methods to characterize the molecular signatures associated with plaque instability in atherosclerotic arteries. Through quantitative proteomic profiling, we depict unique proteome signatures of unstable plaques compared to stable plaques and healthy arteries. Coupled with single-cell RNA-sequencing of leukocytes, we describe the heterodimer complex S100a8/S100a9 as unique to unstable plaque, with neutrophils implicated as the transcriptional drivers of S100a8/a9 expression. We confirm S100a9 expression in human carotid atherosclerotic plaques and we further utilise the TS pre-clinical model to pharmacologically inhibit S100a8/S100a9, resulting in plaque stabilisation. Thus, we establish the TS model as a sophisticated translational tool for the profiling of unstable atherosclerotic plaques and demonstrate that unstable and stable atherosclerosis are highly different disease entities.

2023-05-10 | PXD030857 | Pride

Trem2 Agonist Reprograms Foamy Macrophages to Promote Atherosclerotic Plaque Stability

Project description:To elucidate the impact of Trem2 agonist on aortic gene expression during atherosclerosis.

2024-04-18 | GSE256189 | GEO