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Endogenous hairpins-ir71 targets : Gene profiling of Turnip Crinkle Virus (TCV) siRNA in mutants.

ABSTRACT: gnp06-03_microtrac Endogenous hairpins-ir71 targets What are the genes (including miRNA precursors) that are differentially regulated in a set of viral siRNA in diferent mutants? Lines IR71 KO n°3 and Col-0 were grown on soil until bolting, Flower buds were then harvested and RNA extracted with Qiagen RNeasy Plant mini kit. RNA was stored at -20°C.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE34653 | GEO | 2012/12/31

SECONDARY ACCESSION(S): PRJNA151241

REPOSITORIES: GEO

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Endogenous hairpins-ir71 targets : Gene profiling of Turnip Crinkle Virus (TCV) siRNA in mutants.

Project description:gnp06-03_microtrac Endogenous hairpins-ir71 targets What are the genes (including miRNA precursors) that are differentially regulated in a set of viral siRNA in diferent mutants? Lines IR71 KO nÂ°3 and Col-0 were grown on soil until bolting, Flower buds were then harvested and RNA extracted with Qiagen RNeasy Plant mini kit. RNA was stored at -20Â°C. 3 dye-swap - gene knock out

2012-12-31 | E-GEOD-34653 | biostudies-arrayexpress

Gene profiling of Turnip Crinkle Virus (TCV) siRNA in mutants at generation G1 and G11

Project description:gnp06-03_microtrac - gene profiling of turnip crinkle virus (tcv) sirna in mutants at generation g1 and g11 - What are the genes (including miRNA precursors) that are differentially regulated in a set of viral siRNA in diferent mutants? - Col-0, dcl2/4, dcl3/4 and dcl2/3/4 were grown until rosette state and inoculated with TCV. Ten days post-inoculation the leaves were harvested. The same was made at generation 1 (G1) and 11 (G11).

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Gene profiling of Turnip Crinkle Virus (TCV) siRNA in mutants at generation G1 and G11

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Project description:gnp06-03_microtrac - gene profiling of viral sirna in mutants at generation g1. Gene profiling of Turnip Crinkle Virus (TCV) siRNA in mutants at generation G1. What are the genes (including miRNA precursors) that are differentially regulated in a set of viral siRNA in diferent mutants?

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2012-04-01 | GSE29372 | GEO

gene profiling of viral sirna in mutants at generation g1.

2011-05-24 | E-GEOD-29387 | biostudies-arrayexpress

gene profiling of turnip crinkle virus (tcv) sirna-Gene profiling of Turnip Crinkle Virus (TCV) siRNA in A.thaliana

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Identification of heat induced proteins in young tomato flower buds

Project description:Tomato flowering and fruit set require an optimal temperature of 25/22 ± 2˚C (day/night). When the air temperature reaches to above the optimal range (higher than 30/26˚C; day/night), only a small number of flower buds would develop into mature flowers and produce a reduced number of pollen. This project used the iodoTMT proteomics analysis method to identify heat-induced proteomes in these tomato flower buds.

2022-03-03 | PXD012160 | Pride

Arabidopsis thaliana Wild-type vs. mutant (PcG)

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MicroRNA Clustering Assists Processing of Suboptimal MicroRNA Hairpins Through the Action of the ERH Protein

Project description:Microprocessor initiates processing of microRNAs (miRNAs) from hairpin regions of primary transcripts (pri-miRNAs). Pri-miRNAs often contain multiple miRNA hairpins, and this clustered arrangement can assist processing of otherwise defective hairpins. We find that miR-451, which derives from a hairpin with a suboptimal terminal loop and a suboptimal stem length, accumulates to 40-fold higher levels when clustered with a helper hairpin. This phenomenon tolerates changes in hairpin order, linker lengths, and the identities of the helper hairpin, the recipient hairpin, the linker-sequence, and the RNA polymerase that transcribes the hairpins. It can act reciprocally and need not occur co-transcriptionally. It requires Microprocessor recognition of the helper hairpin and linkage of the two hairpins, yet predominantly manifests after helper-hairpin processing. It also requires Enhancer of Rudimentary Homology (ERH), which copurifies with Microprocessor and can dimerize and interact with other proteins that can dimerize, suggesting a model in which Microprocessor recruits another Microprocessor.

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