Transcriptomics

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Disulfide-Orthogonalized Collagen Hydrogels Balance D-Periodic Assembly and Network Formation for Bone Repair


ABSTRACT: Most protein-based hydrogels used in biomedicine and tissue engineering are derived through self-assembly of natural proteins or cross-linking of protein-polymer conjugates, but the relationship between macroscopic mechanical properties and microscopic protein structure remains poorly understood. Here, we describe de novo collagen hydrogel designs and define the impacts of organization and crosslinking among modular peptide fragments on secondary structure, supramolecular interactions, hierarchical self-assembly, and their influence on microstructure, mechanics, and function of the macroscopic hydrogel. By strategically incorporating disulfide bonds into the adhesive and functional-driver modules of the collagen peptide fragments, we could design supramolecular interactions that promote hydrogel formation while retaining the secondary structure and hierarchical self-assembly of biomimetic D-periodic fibers. Additionally, these synthetic fibers could promote osteoblast differentiation and femoral fracture repair with comparable efficacy to natural collagen in rats. This study provides a bionic hydrogel design framework for tissue engineering, and a valuable biomaterial for potential clinical translation.

ORGANISM(S): Mus musculus

PROVIDER: GSE346834 | GEO | 2026/09/11

REPOSITORIES: GEO

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