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TGIRT-seq-based quantification of snoRNAs across 5 healthy mouse organs

ABSTRACT: Small nucleolar RNAs (snoRNAs) are structured non-coding RNAs that are frequently under-quantified by standard RNA-seq protocols, since their compact secondary structure impedes efficient reverse transcription. TGIRT-seq relies on a thermostable group II intron reverse transcriptase with high fidelity, processivity, and structure-tolerant activity, making it particularly well suited to accurately capture and quantify these RNA species. This dataset presents a TGIRT-seq atlas generated from ribodepleted total RNA of five healthy mouse organs (bone, colon, gonad, liver and lung), enabling accurate snoRNA quantification alongside mRNAs, lncRNAs and other structured non-coding RNAs (e.g. tRNAs), using a genome annotation supplemented with an updated murine snoRNA annotation and tRNA gene annotations from GtRNAdb.

ORGANISM(S): Mus musculus

PROVIDER: GSE347079 | GEO | 2026/09/13

REPOSITORIES: GEO

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