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Genome-wide identification and characterization of RBM22-binding sites.

ABSTRACT: This study aims to identify RBM22-binding sites in P8 wild-type testes. Fresh testes were collected from P8 wild-type, then performed clip-seq .

ORGANISM(S): Mus musculus

PROVIDER: GSE347556 | GEO | 2026/10/01

REPOSITORIES: GEO

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Gene expression in 8d Wild-type and Strs8-cre Rbm22 KO Testis

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Genome-wide maps of RBM22 in P8 wild-type testes and comparison of Pol II traveling ratios (the ratio between gene body and promoter-proximal polymerase) between P8 wild-type and Rbm22 KO testes.

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RBM22 CUT&Tag Profiling of Regenerative (P1) and Non-Regenerative (P8) Neonatal Mouse Cardiomyocytes

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Project description:Purpose: The purpose of this study was to identify chromatin alterations regulated by RBM22 in adult mouse cardiomyocytes (AMCMs) after myocardial infarction (MI). We performed ATAC sequencing on AMCMs isolated from mice post-MI that were infected with either AAV9-cTnT-GFP (control viral vector, AAV9-Control) or AAV9-cTnT-Rbm22-GFP (AAV9-Rbm22). Methods: 8-week-old C57BL/6J mice underwent permanent ligation of the left anterior descending (LAD) coronary artery, followed immediately by intramyocardial injection of either AAV9-Control or AAV9-Rbm22 at the infarct border zone. Adult cardiomyocytes were isolated post-MI using the Langendorff perfusion method. Results: We identified differentially altered chromatin accessibility regions between adult cardiomyocytes injected with the indicated viral vectors post-MI.

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| PRJNA1255616 | ENA

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Project description:Purpose: The purpose of this study is to identify chromatin alterations regulated by RBM22. We performed ATAC-sequencing in primary cultured neonatal mouse cardiomyocytes transfected with control siRNA or Rbm22 siRNA. Methods: Cardiomyocytes were isolated from 1-day-old C57BL/6J mice and cultured in DMEM with 10% fetal bovine serum at 37°C in an atmosphere with 5% CO2. Cardiomyocytes were stained to confirm the expression of cTnT. Cells with purity >97.5% were used for subsequent experiments. Cardiomyocytes were transfected with control siRNA or Rbm22 siRNA for 48 hours. Results: We identified regions of chromatin that were altered between cardiomyocytes transfected with the indicated siRNAs.

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2026-02-12 | GSE295695 | GEO

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