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Central platelet-rich plasma administration promotes weight loss in diet-induced obese mice

ABSTRACT: Platelet-rich plasma (PRP) is widely studied as a regenerative biologic, but whether platelet-derived signals influence central metabolic control remains unknown. Here, we tested whether PRP acts in the brain to reshape hypothalamic cellular responses and energy balance during lipopolysaccharide (LPS)-induced systemic inflammation and diet-induced obesity (DIO). In DIO mice, a single central PRP injection shifted energy balance toward weight loss, reducing body weight, cumulative food intake, relative gonadal white adipose tissue weight, and fasting glucose, while increasing energy expenditure, oxygen consumption, fat utilization, and physical activity. Across both the LPS and DIO models, PRP reduced microglial soma area selectively in the arcuate nucleus (ARC) without changing microglial number. PRP also elicited broad hypothalamic transcriptional changes involving immune and metabolic signaling, extracellular matrix remodeling, and growth responses, rather than a uniform anti-inflammatory signature during DIO. Overall, central PRP evokes a distinctive hypothalamic response accompanied by negative energy balance in established DIO, providing a foundation for identifying active PRP components and the hypothalamic cell populations that mediate these effects.

ORGANISM(S): Mus musculus

PROVIDER: GSE347632 | GEO | 2026/10/01

REPOSITORIES: GEO

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