Transcriptomics

Dataset Information

38

Differential gene expression in human myeloid dendritic cells interacting with different knockout strains of Aspergillus fumigatus in vitro


ABSTRACT: Dendritic cells (DC) are the most important antigen presenting cells and play a pivotal role in host immunity to infectious agents by acting as a bridge between the innate and adaptive immune systems. Myeloid DCs (mDC) were isolated from peripheral blood and co-cultured with conidia (con) and germlings (ks) of Aspergillus fumigatus knockout (mitA, mnt1, rodA) and wildtype (wt) strains for 6 hours at an MOI of 1. RNA was extracted and hybridised to microarrays. Microarrays contain probes for approximately 110 selected human immune-relevant genes, including cytokine and chemokine genes, their receptors and downstream innate immunity genes. Overall design: We used microarrays to detail the gene expression of human myeloid dendritic cells after 6h of co-cultivation with Aspergillus fumigatus wildtype and knockout mutants

INSTRUMENT(S): Operon Oligonucleotide Set Innate Immunity and Aspergillosis 7MM

ORGANISM(S): Homo sapiens  

SUBMITTER: Susanne Elma Kneitz  

PROVIDER: GSE34884 | GEO | 2012-04-17

SECONDARY ACCESSION(S): PRJNA150205

REPOSITORIES: GEO

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