Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

TNF-α treated HUVECs: microRNA negative control VS. miR-181b

ABSTRACT: Transcriptional profiling of TNF-α treated HUVECs comparing cells transfected microRNA negative control with cells transfected with miR-181b.

ORGANISM(S): Homo sapiens

PROVIDER: GSE35030 | GEO | 2012/05/16

SECONDARY ACCESSION(S): PRJNA150861

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

TNF-M-NM-1 treated HUVECs: microRNA negative control VS. miR-181b

Project description:Transcriptional profiling of TNF-M-NM-1 treated HUVECs comparing cells transfected microRNA negative control with cells transfected with miR-181b. Two-condition experiment, miR negative control VS. miR-181b transfected HUVECs. Biological replicates: 4 replicates of comparison of miR negative control VS. miR-181b

2012-05-15 | E-GEOD-35030 | biostudies-arrayexpress

comprehensive chromatin interaction in TNF alpha stimulated HUVECs

Project description:HUVECs were stimulated and samples were prepared after 0 and 30 min. Chromatin interaction mediated by active RNA polymerase II was detected by ChIA-PET. Samples were made afrer 0 and 30 min after TNF alpha stimulation.

2012-10-13 | E-GEOD-41553 | biostudies-arrayexpress

TNF-alpha-stimulated human umblical vein endothelial cells (HUVECs)

Project description:TNF-alpha-stimulated human umblical vein endothelial cells (HUVECs)

| PRJNA501790 | ENA

Genome-wide maps of RNA Polymerase II and p65 localization in HUVECs stimulated with TNF alpha

Project description:TNF alpha is one of the inflammatory mediator and induce genes mainly by transcriptional factor, p65, in endothelial cells. This time, we performed a time course study to detect the change of localization of p65 and Pol II. To identify p65 and Pol II binding sites, we used chromatin immunoprecipitation with deep sequencing (ChIP-seq) of HUVECs treated with or without TNF alpha for 30 mins. Cells were starved before stimulation longer than 16 hours. HUVECs were used within the first 6 passages. For crosslinking, 10 mM of EGS in 50% glacial acetic acid was used for 45 min, followed by 20 min of 1% paraformaldehyde treatmet was used.

2013-01-01 | E-GEOD-34500 | biostudies-arrayexpress

comprehensive chromatin interaction in TNF alpha stimulated HUVECs

Project description:HUVECs were stimulated and samples were prepared after 0 and 30 min. Chromatin interaction mediated by active RNA polymerase II was detected by ChIA-PET.

2012-10-13 | GSE41553 | GEO

Expression data of HUVEC cells after TNF-alpha treatment

Project description:Tumor necrosis factor a (TNF-aplha) is a potent cytokine that orchestrates the inflammatory response by sequentially activating the expression of many genes in cultured human umbilical vein cells (HUVECs). We apply samples collected every 7.5 min to arrays and monitor the growth of several long transcripts. Keywords: time course Before stimulation, HUVECs were serum-starved in EBM-2 (Clonetics) containing 0.5% FBS for 18 h, and then treated with 10 ng/ml TNF (Peprotec) and samples collected every 7.5 min for 3 h.

2007-09-19 | E-GEOD-9036 | biostudies-arrayexpress

RNA-Seq of inflammatory stimulated HUVECs transfected with microRNA-125a-5p or negative control.

Project description:Purpose: In recent years, research has revealed the role of microRNAs (miRs) as important regulators of endothelial function. Notably, miR-125a is upregulated in the blood of patients with acute vascular diseases. Since miRNAs act out their function in networks, we aimed at investigating the presence of a miR-125a-related network regulating the endothelial barrier. Method: We investigated transcriptional changes of human umbilical cord endothelial cells (HUVEC) after miR-125a overexpression in an acute inflammatory in-vitro setting using Next Generation Sequencing. Briefly, primary HUVECs from five different donors were transfected either with hsa-miR-125a-5p or negative control (NC). After transfection HUVECs were cultivated for 18 hours and stimulated with TNF (25 ng/ml) for additional 6 hours. We compared the following groups: NC (18047-0001, 18047-0002, 18047-0003, 18047-0004, 18047-0005) versus miR125a: (18047-0006, 18047-0007, 18047-0008, 18047-0009, 18047-0010) Results: MiR-125a overexpression in inflammatory activated HUVECs significantly altered gene expression of 468 genes (FC: +1.25 to -1.25 p<0.01). We found eight potential miR-125a direct target genes involved in endothelial barrier function.

2022-02-07 | GSE196161 | GEO

Hi-C analysis in TNF-alpha-stimulated human umblical vein endothelial cells (HUVECs)

Project description:Hi-C analysis in TNF-alpha-stimulated human umblical vein endothelial cells (HUVECs)

| PRJNA497537 | ENA

Global expression analysis of miR-32/455/181a/181b overexpression or PI3K knockdown in KYSE30 cells

Project description:Analysis of the effect of the miRNA set (miR-32/455/181a/181b) on global gene expression. The hypothesis tested was that overexpression of these miRNAs would produce changes in gene expression similar to changes caused by PI3K knockdown. Data provided insight into genes that are disrupted with either individual miRNA and siRNA or the combination of miRNAs or siRNAs. Total RNA was isolated from KYSE30 cells transfected with either miRNA or siRNA for global expression analysis.

2012-12-29 | E-GEOD-43195 | biostudies-arrayexpress

Transcription profiling of Homo Sapiens Human Umbilical Endothelial Cells normal vs treated with TNF to investigate effect of TNF-alpha on microRNAs levels

Project description:Effect of TNF-alpha on microRNAs levels in Human Umbilical Endothelial Cells (HUVECs). HUVEC that were treated or not for 2 or 24 hours with TNF (10 ng/ml). Duplicate samples (1 or 2) of two different isolations of HUVEC (A or B)

2009-12-01 | E-MTAB-150 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data