H3K27me3 and total H3 ChIP-seq in stage 9, 11, and 12 Drosophila melanogaster embryos

ABSTRACT: In an associated manuscript, we measured by ChIP-seq the genome-wide distribution of Histone H3 trimethylated at Lysine 27 (H3K27me3) and total Histone H3 in Drosophila embryos at three developmental stages following the maternal-to-zygotic transition: stage 9 (~NC14 + 2h), stage 11 (~NC14 + 4h), and stage 12 (~NC14 + 6h). These measurements extend a prior time course of H3K27me3 establishment during nuclear cycle 14 (NC14; GSE299311) and show that, after H3K27me3 appears local to Polycomb Response Elements (PREs) in mid-NC14, the modification continues to spread outward from these nucleation sites over the following ~6 hours. In the associated manuscript, these data are used to quantify the in vivo rate of H3K27me3 spreading and to benchmark a reaction-diffusion model of de novo Polycomb domain establishment by PRC2.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE352458 | GEO | 2026/10/29

REPOSITORIES: GEO