Evaluation of circular guide RNA capture in Parse Evercode v3 single-cell profiling of mixed human induced pluripotent stem cells and K562 cells

ABSTRACT: Circular capture Perturb-seq directly detects guide RNA transcripts after split-pool cellular barcoding. We evaluated its compatibility with Parse Evercode WT v3 using separately fixed human induced pluripotent stem cells and K562 cells carrying distinct pooled guide libraries. Fixed populations were mixed before barcoding, and Round 1 reverse transcription used CS1, DC1, both guide-specific primers or no guide-specific primer. Matched transcriptome and guide measurements were used to assess guide recovery, guide-pool specificity, background capture, transcriptome recovery and sequencing saturation. The deposited integrated object reproduces Figures S2i, S2j, S2l, S2m and S2n of Mapping the regulatory wiring of endothelial cell differentiation reveals a critical role for Hippo mechanosignaling.

ORGANISM(S): Homo sapiens

PROVIDER: GSE352893 | GEO | 2026/11/02

REPOSITORIES: GEO