Genome-wide analysis of gene expression after tyrosine or methionine/cysteine deprivation in HeLa cells

ABSTRACT: Limitation of essential amino acids, such as tyrosine or methionine/cysteine, causes upregulation of exogenous integrated transgene expression in mammalian cells. This phenomenon is mediated by histone acetylation and chromatin remodelling, since histone deacetylase (HDAC) inhibitors reproduce starvation-induced transgene upregulation and chromatin immunoprecipitation analysis of amino acid-deprived cells reveals significant changes in total core histones detectable at the CMV promoter. Expression profiling of HeLa cells starved for 5 days in medium without tyrosine or methionine/cysteine provides important information on the cellular response to amino acid deprivation and suggests the involvement of HDAC4 (class II HDAC) in transgene derepression during amino acid starvation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE35323 | GEO | 2012/07/24

SECONDARY ACCESSION(S): PRJNA151551

REPOSITORIES: GEO