MSR-seq profiling of commercial E. coli tRNA following in vitro Cas13b6 cleavage

ABSTRACT: MSR-seq was used to profile commercial Escherichia coli tRNA (New England Biolabs) following in vitro reactions containing wild-type Cas13b6, H104A or the catalytically inactive dHEPN1 variant, together with an enzyme-free input-stock control. For each source preparation, MesoRNA prepared matched Tris-deacylated and periodate-treated (IO4) libraries. The dataset includes sequencing reads and vendor-provided alignment, abundance and basewise modification-signature outputs. This separate MSR-seq cohort complements the RNA-end and cellular RNA-seq data in related study GSE348432. A SuperSeries linking the two studies is planned; no SuperSeries accession has been assigned.

ORGANISM(S): Escherichia coli

PROVIDER: GSE353609 | GEO | 2026/11/09

REPOSITORIES: GEO