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Transcriptome analysis of differentiating normal and leukemic erythroid progenitors

ABSTRACT: We compared the transcriptomes of differentiating cultures of ES cell derived erythroid progentor cells (ES-EP) and murine erythroleukemia (MEL) cells stably transfected with GATA-1 fused to ER.

ORGANISM(S): Mus musculus

PROVIDER: GSE35384 | GEO | 2012/02/27

SECONDARY ACCESSION(S): PRJNA156067

REPOSITORIES: GEO

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Transcriptome analysis of differentiating normal and leukemic erythroid progenitors

Project description:We compared the transcriptomes of differentiating cultures of ES cell derived erythroid progentor cells (ES-EP) and murine erythroleukemia (MEL) cells stably transfected with GATA-1 fused to ER. RNA was isolated from duplicate differntiating cultures of MEL and ES-EP using Affymetrix GeneChip Mouse Gene 1.0 ST.

2012-02-27 | E-GEOD-35384 | biostudies-arrayexpress

Transcriptome analysis of normal erythroid progenitors and erythroleukemia cells

Project description:We compared the transcriptomes of ES cell derived erythroid progentor cells (ES-EP) and murine erythroleukemia (MEL) cells stably transfected with Gata-1 fused to ER.

2011-06-23 | GSE21949 | GEO

Transcriptome analysis of normal erythroid progenitors and erythroleukemia cells

Project description:We compared the transcriptomes of ES cell derived erythroid progentor cells (ES-EP) and murine erythroleukemia (MEL) cells stably transfected with Gata-1 fused to ER. RNA was isolated from duplicate proliferating cultures of MEL and ES-EP using Affymetrix GeneChip Mouse Gene 1.0 ST.

2011-06-23 | E-GEOD-21949 | biostudies-arrayexpress

GATA-1 in proliferating and differentiating murine ES cell derived erythroid progenitors

Project description:This SuperSeries is composed of the following subset Series: GSE35379: Genome-wide occupancy map of GATA-1 in proliferating and differentiating murine ES cell derived erythroid progenitors (ES-EP) GSE35384: Transcriptome analysis of differentiating normal and leukemic erythroid progenitors Refer to individual Series

2012-02-27 | E-GEOD-35385 | biostudies-arrayexpress

Analysis of gene expression in MEL cell before and after erythroid-like differentiation.

Project description:To promote the development and understanding of the in vitro erythroleukemia model, we analyzed the transcriptomes of mouse erythroleukemia (MEL) cells prior to and after erythroid-like differentiation induced by dimethyl sulfoxide (DMSO). A total of 348 protein-coding genes, including many known erythroid-enriched genes such as hemoglobin and heme synthesis genes, were upregulated upon erythroid-like induction in MEL cells

2019-10-01 | GSE133343 | GEO

GATA-1-regulated erythroid microRNAs

Project description:We employed a gene complementation strategy combined with microarray screening to identify miRNAs involved in the formation of erythroid (red blood) cells. To search for GATA-1-regulated erythroid miRNAs, we used the Gata-1– erythroblast line G1E. These cells proliferate in culture as immature erythroid precursors and undergo terminal maturation when GATA-1 activity is restored. G1E-ER4 is a sub-line stably expressing an estrogen-activated form of GATA-1 (GATA-1 fused to the ligand binding domain of the estrogen receptor). Treatment of G1E-ER4 cells with estradiol induces a GATA-1-regulated program of gene expression with concomitant cellular maturation. We used a microarray to evaluate the expression of 292 different miRNAs in G1E-ER4 cells at 0 versus 24 hours after GATA-1 activation. Affymetrix gene expression profiling has previously been deposited (GEO accession no. GSE628). Keywords: microRNA analysis of a cell-line model of erythroid maturation

2008-02-05 | GSE10134 | GEO

Erythroid Differentiation: G1E Model

Project description:G1E cells are a Gata-1 erythroid-committed cell line derived from targeted disruption of Gata-1 in embryonic stem cells. The ER4 subclone contains an inducible form of Gata-1 (Gata-1-ER, Gata-1 fused to the estradiol receptor ligand binding domain). We performed transcriptome analysis using this cell line. Estradiol was added to culture medium triggering synchronous and homogenous differentiation. At various time points, RNA was sampled and analyzed using the Affymetrix MG-U74Av2 platform. Three biological replicas (A,B, and C) were performed. The thirty hour time course corresponds to development from the late BFU-E stage through the orthochromatic erythroblast stage. Keywords = Gata1 Keywords = erythroid Keywords = mouse Keywords: time-course

2004-02-01 | GSE628 | GEO

GATA-1-regulated erythroid microRNAs

2008-02-05 | E-GEOD-10134 | biostudies-arrayexpress

Ldb1-nucleated transcription complexes function as primary mediators of erythroid gene activation

Project description:We used ChIP-Seq to map Ldb1, Scl and Gata1 binding sites in mouse total bone marrow cells. Together with functional studies comparing gene expression in Murine Erythroleukemia (MEL) cells expressing Ldb1 shRNA or control shRNA and bioinformatics analysis, we systematically determined the transcriptional program controlled by Ldb1 complexes in erythropoiesis. This represents the ChIP-Seq component of the study only To evaluate the role of Ldb1complexes in erythroid gene activation

2013-04-29 | E-GEOD-42843 | biostudies-arrayexpress

TAF10 interacts with GATA1 transcription factor and controls mouse erythropoiesis

Project description:The stoichiometries of TFIID and SAGA (TAF10-containing complexes) have been quantified in mouse and human erythroid cells. TAF10 immunoprecipitations (IPs) have been carried out in erythroid cells at different stages of differentiation and development followed by quantitative mass spectrometry (MS). Interactions of TFIID and SAGA with several transcription factors and specifically with GATA-1 have been identified. GATA-1 immunoprecipitation (IP) in MEL cells also identified the reverse interactions with subunits of the TFIID and SAGA after MS analysis.

2015-04-21 | PXD000729 | Pride

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