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Gene expression data from isolated white winter wheat embryos at physiological maturity, environment 2 of 2.

ABSTRACT: Gene expression was measured in isolated wheat embryos at physiological maturity. The gene expression measurements were done across a doubled haploid white winter wheat population between the variety Cayuga and the variety Caledonia (CxC). Data can be used for mapping gene expression QTL (eQTL). These data are from the second (Ketola 2005) of two field environments. Different target labeling was used so the environments are contained in two separate series.

ORGANISM(S): Triticum aestivum

PROVIDER: GSE35407 | GEO | 2012/02/01

SECONDARY ACCESSION(S): PRJNA152797

REPOSITORIES: GEO

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Gene expression data from isolated white winter wheat embryos at physiological maturity, environment 1 of 2.

2012-02-01 | GSE35300 | GEO

Gene expression data from isolated white winter wheat embryos at physiological maturity, environment 2 of 2.

Project description:Gene expression was measured in isolated wheat embryos at physiological maturity. The gene expression measurements were done across a doubled haploid white winter wheat population between the variety Cayuga and the variety Caledonia (CxC). Data can be used for mapping gene expression QTL (eQTL). These data are from the second (Ketola 2005) of two field environments. Different target labeling was used so the environments are contained in two separate series. The design was a two-channel dye-swap reference design with bulked total RNA from pre-anthesis wheat spikes serving as the reference. Normalization was done using the TIGR MIDAS suite using blockwise LOWESS smoothing and variance normalization. Between slide variance normalization was also done for all slides so direct comparisons could be made. Dye-swap was submitted unmerged to facilitate flexibility in downstream processing.

2012-02-01 | E-GEOD-35407 | biostudies-arrayexpress

Gene expression data from isolated white winter wheat embryos at physiological maturity, environment 1 of 2.

Project description:Gene expression was measured in isolated wheat embryos at physiological maturity. The gene expression measurements were done across a doubled haploid white winter wheat population between the variety Cayuga and the variety Caledonia (CxC). Data can be used for mapping gene expression QTL (eQTL). These data are from the first (Helfer 2004) of two field environments. Different target labeling was used so the environments are contained in two separate series. The design was a two-channel dye-swap reference design with bulked total RNA from pre-anthesis wheat spikes serving as the reference. Normalization was done using the TIGR MIDAS suite using blockwise LOWESS smoothing and variance normalization. Between slide variance normalization was also done for all slides so direct comparisons could be made. Dye-swap was submitted unmerged to facilitate flexibility in downstream processing.

2012-02-01 | E-GEOD-35300 | biostudies-arrayexpress

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Identification and Temporal Expression Analysis of Conserved and Novel MicroRNAs in the Leaves of Winter Wheat Grown in the Field

Project description:MicroRNAs (miRNAs) are small non-coding RNAs that regulate target mRNAs by inducing degradation or preventing translation of their target mRNAs. Winter wheat, Triticum aestivum., is an important crop plant, yet there are only a few studies on the association of miRNAs and growth and development of winter wheat grown in the field. Here we carried out experimental analysis of miRNAs in wheat leaves by analyzing small RNA profiles at different growth stages.

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Light effect on the hormonal and transcriptional status of winter and spring wheat plants during cold hardening

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