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Genome-wide analysis of human macrophages stimulated with IL-4 (20ng/ml) (Illumina)

ABSTRACT: Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4.

ORGANISM(S): Homo sapiens

PROVIDER: GSE35433 | GEO | 2012/09/29

SECONDARY ACCESSION(S): PRJNA156051

REPOSITORIES: GEO

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Genome-wide analysis of human macrophages stimulated with IL-4 (10ng/ml) (Illumina)

2012-09-29 | GSE35434 | GEO

Genome-wide analysis of human macrophages stimulated with IL-4 (10ng/ml) (Illumina)

Project description:Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from human monocyte derived macrophages upon exposure to 10 ng/ml of IL-4 for 48 hours. Monocytes were induced to mature into macrophages using X Vivo 10 medium supplemented with 1% autologous serum.

2012-09-29 | E-GEOD-35434 | biostudies-arrayexpress

Genome-wide analysis of human macrophages stimulated with IL-4 (20ng/ml) (Illumina)

Project description:Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from human monocyte derived macrophages upon exposure to 20 ng/ml of IL-4 for 24 hours. Monocytes were induced to mature into macrophages using X Vivo 10 medium supplemented with 1% autologous serum.

2012-09-29 | E-GEOD-35433 | biostudies-arrayexpress

Genome-wide analysis of mouse macrophages stimulated with IL-4 (bone marrow macrophages) (Affymetrix)

Project description:Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from bone marrow derived macrophages upon exposure to 20 ng/ml of IL-4 for 18 hours. Bone marrow derived macrophages were stimulated with the Th2 cytokine IL-4, for RNA extraction and hybridization on Affymetrix microarrays.

2012-09-29 | E-GEOD-35435 | biostudies-arrayexpress

Genome-wide analysis of mouse macrophages stimulated with IL-4 (Biogel and thioglycollate macrophages) (Affymetrix)

Project description:Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from Thioglycollate and Biogel elicited peritoneal macrophages exposed to 20 ng/ml of IL-4 for 18 hours. Biogel and thioglycollate elicited macrophages were stimulated with the Th2 cytokine IL-4, for RNA extraction and hybridization on Affymetrix microarrays.

2012-09-29 | E-GEOD-35436 | biostudies-arrayexpress

Genome-wide analysis of mouse macrophages stimulated with IL-4 (bone marrow macrophages) (Affymetrix)

Project description:Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from bone marrow derived macrophages upon exposure to 20 ng/ml of IL-4 for 18 hours.

2012-09-29 | GSE35435 | GEO

Genome-wide analysis of mouse macrophages stimulated with IL-4 (Biogel and thioglycollate macrophages) (Affymetrix)

Project description:Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from Thioglycollate and Biogel elicited peritoneal macrophages exposed to 20 ng/ml of IL-4 for 18 hours.

2012-09-29 | GSE35436 | GEO

Proteome of BaF3-mEpoR cells stimulated with 5 U/ml Epo, 10 ng/ml IL-3 or left untreated

Project description:The proteome of BaF3-mEpoR cells stimulated with 5 U/ml Epo, 10 ng/ml IL-3 or left untreated were analyzed by label free LC-MS/MS.

2022-09-28 | PXD035181 | Pride

High salt reduces the activation of IL-4+IL-13 stimulated macrophages

Project description:High salt (NaCl) intake is a major risk factor for cardiovascular disease; high NaCl has also been shown to be a powerful modulator of pro-inflammatory immune cell polarization. To investigate the effect of high NaCl on the polarization of M(IL-4+IL-13) macrophages we performed gene expression analysis by microarray and genome wide histone modification (H3K4me3 and H4ac) analysis by ChIP-seq of murine bone marrow derived macrophages. Macrophages were polarized with recombinant IL-4 and IL-13 in vitro in the presence or absence of an additional 40mM of NaCl in the culture medium for 24h before analysis.

2015-02-07 | E-MTAB-3140 | biostudies-arrayexpress

High salt reduces the activation of IL-4+IL-13 stimulated macrophages

2014-12-16 | E-MTAB-3163 | biostudies-arrayexpress

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