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Dataset Information

47

ChIP-Seq of Sox2 and Brn2 in ESCs, NPCs, and differentiating ESCs


ABSTRACT: We analyzed the genome-wide binding of Sox2 and POU factor partner factors, Oct4 in ESCs (using published datasets PMID:18692474 and GSM307137, GSM307154, GSM307155) and Brn2 in NPCs. We found that Sox2 and Oct4 co-occupied a large subset of promoters and enhancers in ESCs, but that Sox2 and Brn2 co-occupy predominantly enhancers. Further, we overexpressed Brn2 in differentiating ESCs and showed that ectopic Brn2 recruited Sox2 to NPC-specific targets, resulting in skewed differentiation towards the neural lineage. Overall design: Examination of transcription factor binding in ESCs, NPCs, and differentiating ESCs by ChIP-Seq.

REANALYSIS of: GSE11724

INSTRUMENT(S): Illumina Genome Analyzer II (Mus musculus)

ORGANISM(S): Mus musculus  

SUBMITTER: Michael Lodato  

PROVIDER: GSE35496 | GEO |

SECONDARY ACCESSION(S): PRJNA152295

REPOSITORIES: GEO

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