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Identification of the Bacteroides fragilis EcfO regulon 1

ABSTRACT: Genome expression study of Bacteroides fragilis ATCC25285 strain containing the EcfO gene constitutively expressed from plasmid pFD340

ORGANISM(S): Bacteroides fragilis NCTC 9343

PROVIDER: GSE35536 | GEO | 2013/01/01

SECONDARY ACCESSION(S): PRJNA156093

REPOSITORIES: GEO

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Identification of the Bacteroides fragilis EcfO regulon 1

Project description:Genome expression study of Bacteroides fragilis ATCC25285 strain containing the EcfO gene constitutively expressed from plasmid pFD340 Two chip study with 5 technical replicates on each chip comparing the EcfO overexpressing strain to an empty vector control in the wild type strain.

2013-01-01 | E-GEOD-35536 | biostudies-arrayexpress

Identification of the Bacteroides fragilis EcfO regulon 2

2013-01-01 | GSE35537 | GEO

Identification of the Bacteroides fragilis EcfO regulon 3

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Identification of the Bacteroides fragilis EcfO regulon 2

Project description:Genome expression study of Bacteroides fragilis strain 638R comparing a EcfO null mutant with an empty vector to a anti-EcfO null mutant containing the EcfO gene on a plasmid under the control of an IPTG inducible promoter. Two chip study with 8 technical replicates on each chip comparing the EcfO overexpressing strain in an anti-EcfO mutant to an empty vector control in a EcfO null mutant.

2013-01-01 | E-GEOD-35537 | biostudies-arrayexpress

Role of the Bacteroides fragilis EcfO regulon during oxidative stress.

2013-02-06 | GSE39231 | GEO

Identification of the Bacteroides fragilis EcfO regulon 3

Project description:Genome expression study of Bacteroides fragilis strain 638R comparing a EcfO/anti-EcfO null mutant with an empty vector to a EcfO/anti-EcfO null mutant containing the EcfO gene on a plasmid under the control of a maltose inducible promoter. Two chip study with 8 technical replicates on each chip comparing the EcfO overexpressing strain in a EcfO/anti-EcfO mutant to an empty vector control in the same mutant background.

2013-01-01 | E-GEOD-35538 | biostudies-arrayexpress

Role of the Bacteroides fragilis EcfO regulon during oxidative stress.

Project description:Genome expression study of Bacteroides fragilis strain 638R comparing an ecfO null mutant with an empty vector to a reo (anti-ecfO) null mutant containing the ecfO gene on a plasmid under the control of an IPTG inducible promoter. Seven chip study with 8 technical replicates (i.e. Each probe replicated 8 times on the chip) on each chip comparing the ecfO overexpressing strain in a reo (anti-ecfO) mutant to an empty vector control in an ecfO null mutant.

2013-02-06 | E-GEOD-39231 | biostudies-arrayexpress

Expression analysis of Bacteroides fragilis NCTC 9343 delta-ungD1 delta-ungD2

Project description:Investigation of whole genome gene expression level changes in a Bacteroides fragilis NCTC 9343 delta-ungD1 delta-ungD2 double mutant compared to the wild-type strain. Keywords: expression analysis A six chip study using total RNA recovered from three separate wild-type cultures of Bacteroides fragilis NCTC 9343 and three separate cultures of a double mutant strain, Bacteroides fragilis NCTC 9343 delta-ungD1 delta-ungD2, in which ungD1 (BF1706) and ungD2 (BF2848) are deleted. Each chip measures the expression level of 4,302 genes from Bacteroides fragilis NCTC 9343 and the associated plasmid pBF9343 with fourteen 24-mer probe pairs (PM/MM) per gene, with three-fold technical redundancy.

2010-04-15 | E-GEOD-7450 | biostudies-arrayexpress

Expression analysis of Bacteroides fragilis NCTC 9343 delta-gmd-fcl delta-fkp and B. fragilis NCTC 9343 delta-lfg

Project description:Investigation of whole genome gene expression level changes in a Bacteroides fragilis NCTC 9343 delta-gmd-fcl delta-fkp mutant strain and a Bacteroides fragilis NCTC 9343 delta-lfg mutant strain, each as compared to the wild-type strain. The mutations engineered into these strains interfere with B. fragilis protein glycosylation.

2010-08-06 | GSE12440 | GEO

Expression analysis of Bacteroides fragilis NCTC 9343 delta-ungD1 delta-ungD2 delta-PSH acapsular mutant

Project description:Investigation of whole genome gene expression level changes in a Bacteroides fragilis NCTC 9343 delta-ungD1 delta-ungD2 delta-PSH triple mutant, compared to the wild-type strain. The mutations engineered into this strain render it acapsular. The mutants analyzed in this study are further described in Coyne, M. J., M. Chatzidaki-Livanis, L. C. Paoletti, and L. E. Comstock. 2008. Role of glycan synthesis in colonization of the mammalian gut by the bacterial symbiont Bacteroides fragilis. PNAS 105(35):13098-13103 (PID 18723678). A six chip study using total RNA recovered from three separate wild-type cultures of Bacteroides fragilis NCTC 9343 and three separate cultures of a triple mutant strain, Bacteroides fragilis NCTC 9343 delta-ungD1 delta-ungD2 delta-PSH, in which ungD1 (BF1706), ungD2 (BF2848), and six genes (BF3454 through BF3459) of the PSH capsular polysaccharide locus are truncated or deleted entirely. Each chip measures the expression level of 4,302 genes from Bacteroides fragilis NCTC 9343 and the associated plasmid pBF9343 with fourteen 24-mer probe pairs (PM/MM) per gene, with three-fold technical redundancy.

2008-09-02 | E-GEOD-11268 | biostudies-arrayexpress

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