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The role of FOG-1 in GATA-1 transcriptional regulation of megakaryocyte differentiation

ABSTRACT: We explored the role of FOG-1 in GATA-1 transcriptional regulation of megakaryocyte differentiation through expression of wild-type GATA-1 and the FOG-binding mutant of GATA-1 (GATA-1^V205G) in G1ME cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE35695 | GEO | 2012/03/05

SECONDARY ACCESSION(S): PRJNA155849

REPOSITORIES: GEO

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The role of FOG-1 in GATA-1 transcriptional regulation of megakaryocyte differentiation

Project description:We explored the role of FOG-1 in GATA-1 transcriptional regulation of megakaryocyte differentiation through expression of wild-type GATA-1 and the FOG-binding mutant of GATA-1 (GATA-1^V205G) in G1ME cells. G1ME cells were derived from Gata1-null mouse ES cells and have both megakaryocyte and erythrocyte differentiation potential upon reconstitution of GATA-1 expression (Stachura 2006). HA-tagged wild-type or mutant GATA-1 were expressed in G1ME cells grown in TPO via retroviral transductions. The cells were sorted for GFP positivity 68 hours post-transduction and then were allowed to recover in normal growth medium for 4h. Total RNA was then isolated using RNeasy kit from Qiagen 72 hours post-transduction.

2012-03-05 | E-GEOD-35695 | biostudies-arrayexpress

Genome-wide analysis of the role of FOG-1 in GATA-1 chromatin occupancy

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2012-07-30 | E-GEOD-35644 | biostudies-arrayexpress

Genome-wide analysis of the role of FOG-1 in GATA-1 chromatin occupancy

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Genome-wide analysis of the role of FOG-1 in GATA-1 chromatin occupancy

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Project description:Downregulation of GATA-2 in megakaryocytic cells G1ME caused cell cycle arrest. To identify GATA-2 target genes, we transduced G1ME cells with control vector or a vector expressing shRNA specific for mouse GATA-2. The transduced cells were purified by sorting and the RNA were used for microarry.

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Project description:Identification of genes regulated by GATA-1 independent of the cofactor FOG-1. Experiment Overall Design: A conditionally activated FOG-1-binding defective mutant of GATA-1, ER-GATA-1(V205G), was expressed in GATA-1-null G1E cells. Transcipt levels were compared in cells untreated or treated with estradiol to activate the GATA-1 mutant.

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Cofactor-mediated Restriction of GATA-1 Chromatin Occupancy Coordinates Lineage-specific Gene Expression

Project description:This SuperSeries is composed of the following subset Series: GSE35644: Genome-wide analysis of the role of FOG-1 in GATA-1 chromatin occupancy GSE35695: Differential gene regulation by disease-associated mutants of GATA-1 during megakaryocyte differentiation Refer to individual Series

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Transcription profiling of megakaryocyte cells from Gata-1 knock out mice and wild type mice to study the differential requirements for the activation domain and FOG-interaction surface of GATA-1 in megakaryocyte gene expression and development

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