Transcriptomics

Dataset Information

49

Whole-genome expression data from purified larval Drosophila LNv pacemaker neurons


ABSTRACT: We generated whole genome expression profiles from a homogeneous population of purified pacemaker neurons (ventral Lateral Neurons, LNvs) from wild type and clock mutant Drosophila. The study identifes a group of genes whose expression is highly enriched in LNvs compared to other neurons; and a second group of genes rhythmically expressed in LNvs in a clock-dependent manner. Overall design: Drosophila larval brains (L3 stage) were taken from a 12:12hr light entrainment regime and dissected at ZT-3 or ZT-15. Larvae contained either Pdf-Gal4:UAS-GFP transgenes or Pdf-RFP to label the 8 LNvs pacemaker neurons per brain, allowing purification by flow cytometry. Brains were dissociated and LNvs purified by FACS, followed by RNA amplification and hybridization on Affymetrix microarrays.

REANALYSED by: GSE119128

INSTRUMENT(S): [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Marc Ruben 

PROVIDER: GSE35752 | GEO |

SECONDARY ACCESSION(S): PRJNA152095

REPOSITORIES: GEO

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