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CGH analysis of AG-haESCs (androgenetic haploid embryonic stem cells)

ABSTRACT: The genomic DNA sample of AG-haESCs were compared to the C57BL/6J male mouse kidney by comparative genomic hybridization. The data confirmed that the haploid cells sustained genome integrity.

ORGANISM(S): Mus musculus

PROVIDER: GSE35786 | GEO | 2012/04/27

SECONDARY ACCESSION(S): PRJNA155909

REPOSITORIES: GEO

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CGH analysis of AG-haESCs (androgenetic haploid embryonic stem cells)

Project description:The genomic DNA sample of AG-haESCs were compared to the C57BL/6J male mouse kidney by comparative genomic hybridization. The data confirmed that the haploid cells sustained genome integrity. The analysis was performed on a NimbleGen Mouse CGH 3x720K Whole-Genome Tiling Array to analyse the copy number variations in AG-haESCs, and the genomic DNA of C57BL/6J male mouse kidney was used as control, which had the same background with haploid ESCs.

2012-04-26 | E-GEOD-35786 | biostudies-arrayexpress

DNA methylation profiling in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

Project description:We generated RRBS data to analyse the DNA methylation profiling among WT-AG-haESCs, DKO-AG-haESCs and round spermatids, we found deletion of H19 and Gtl2 DMRs do not change the methylation patterns in AG-haESCs base on all detected CpG sites.

2015-06-29 | GSE60075 | GEO

Gene expression analysis in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

2015-06-29 | GSE60072 | GEO

CGH analysis of monkey PG-haESCs (parthenogenic haploid embryonic stem cells)

2013-06-26 | GSE43979 | GEO

DNA methylation profiling in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

2015-06-29 | E-GEOD-60075 | biostudies-arrayexpress

CGH analysis of monkey PG-haESCs (parthenogenic haploid embryonic stem cells)

Project description:The genomic DNA sample of monkey PG-haESCs were compared to the female adipose cells by comparative genomic hybridization. The data confirmed that these haploid cells sustained genome integrity. The analysis was performed on a Agilent aCGH G3 Rhesus Macaque 4x180K array to analyse the copy number variations in monkey PG-haESCs, and the genomic DNA of femal monkey adipose was used as control, which had the same background with haploid ESCs.

2013-06-26 | E-GEOD-43979 | biostudies-arrayexpress

Gene expression analysis in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

Project description:RNA seq result shows that WT-AG-haESCs and DKO-AG-haESCs samples are clustered together using hierarchical cluster both in the all expression genes and imprinting genes. This suggests that DKO of DMRs of H19 and Gtl2 do not change the overall gene expression patterns in AG-haESCs. We used round spermatids as control. Using RNA-seq, profile of all the expression genes and imprinting genes beteween different samples were analysed.

2015-06-29 | E-GEOD-60072 | biostudies-arrayexpress

Mouse Androgenetic Haploid Embryonic Stem Cells Support Targeted Gene Transmission to Progeny

Project description:This SuperSeries is composed of the following subset Series: GSE35785: mRNA expression data from AG-haESC, E14 and MEF GSE35786: CGH analysis of AG-haESCs (androgenetic haploid embryonic stem cells) Refer to individual Series

2012-04-26 | E-GEOD-35787 | biostudies-arrayexpress

Epigenetic integrity of paternal imprints enhances the developmental potential of androgenetic haploid embryonic stem cells

Project description:The use of two inhibitors of Mek1/2 and Gsk3β (2i) promotes the generation of mouse diploid and haploid embryonic stem cells (ESCs) from the inner cell mass of biparental and uniparental blastocysts, respectively. However, a system enabling long-term maintenance of imprints in ESCs has proven challenging. Here, we report that usage of a two-step a2i (alternative two inhibitors of Src and Gsk3β, TSa2i) derivation/culture protocol results in the establishment of androgenetic haploid ESCs (AG-haESCs) with stable DNA methylation at paternal DMRs (differentially DNA methylated regions) up to passage 60 that can efficiently support generating mice upon oocyte injection. We also show coexistence of H3K9me3 marks and ZFP57 bindings with intact DMR methylations. Furthermore, we demonstrate that TSa2i-treated AG-haESCs are a heterogeneous cell population regarding paternal DMR methylation. Strikingly, AG-haESCs with late passages display increased paternal-DMR methylations and improved developmental potential compared to early-passage cells, in part through the enhanced proliferation of H19-DMR hypermethylated cells. Together, we establish AG-haESCs that can long-term maintain paternal imprints.

2022-01-26 | GSE168447 | GEO

mRNA expression data from AG-haESC, E14 and MEF

Project description:Haploid cells are amenable for genetic analysis because they contain only one set of chromosomes.Here,we report the derivation of haESCs from androgenetic blastocysts. These cells, which we designated AG-haESCs, express classical ESC markers, are pluripotent, and contribute to various tissues including the germline upon injection into diploid blastocysts. We used microarrays to compare the gene expression levels among androgenetic haploid embryonic stem cell lines(AG-haESC) E14 and male mouse embryonic fibroblasts (MEFs) and identified that most paternally imprinted genes were down-regulated and the maternally imprinted genes were up-regulated.

2012-04-27 | GSE35785 | GEO

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