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High throughput sequencing of the mouse transcriptome within the VTA following exposure to emotional stress.

ABSTRACT: Using a novel stress paradigm, we generated emotionally stressed, physically stressed, and control animals. We measured changes in mRNA expression using Illumina HiSeq2000 to obtain high-depth sequencing. Interestingly, we observed a number of significantly up- and down-regulated mRNAs. By identifying mRNAs that were regulated similarly in both emotionally and physically stressed mice, we hope to provide a list of potentially novel targets for therapeutic intervention in mood disorder.

ORGANISM(S): Mus musculus

PROVIDER: GSE36005 | GEO | 2012/08/21

SECONDARY ACCESSION(S): PRJNA151701

REPOSITORIES: GEO

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High throughput sequencing of the mouse transcriptome within the VTA following exposure to emotional stress.

Project description:Using a novel stress paradigm, we generated emotionally stressed, physically stressed, and control animals. We measured changes in mRNA expression using Illumina HiSeq2000 to obtain high-depth sequencing. Interestingly, we observed a number of significantly up- and down-regulated mRNAs. By identifying mRNAs that were regulated similarly in both emotionally and physically stressed mice, we hope to provide a list of potentially novel targets for therapeutic intervention in mood disorder. Examination of the transcriptome within the VTA in emotionally and physically (both susceptible and resilient) stressed C57BL/6J male mice.

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Hypothermic oxygenated machine perfusion influences the immunogenicity of donor livers in humans

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| S-EPMC8804001 | biostudies-literature

Nicotinic receptors promote susceptibility to social stress in female mice linked with neuroadaptations within VTA dopamine neurons.

Project description:There are about twice as many women as men who experience depression during their lifetime. Although life circumstances and especially exposure to stressful situations constitute a major risk factor to develop depression, the underlying mechanisms have yet to be unraveled. We employed the chronic social defeat procedure to elicit depressive-like symptoms in females and ketamine to validate the model. We performed ex-vivo patch clamp recordings to assess cellular adaptations and used pharmacological agents to dissect these deregulations. Chronic social defeat exposure triggers a hyperactivity of VTA putative dopamine (DA) neurons in females susceptible to stress but not resilient ones. This hyperactivity was fully reversed by a single administration of ketamine. In virally-identified brain circuits of both susceptible and resilient females, we found a hypercholinergic tone to the VTA arising from the laterodorsal tegmentum. Application of puffs of nicotine revealed a decreased sensitivity of DA neurons in resilient mice when compared to naive or susceptible ones. The in vivo acute administration of the positive allosteric modulator for α7 nicotinic acetylcholine receptors (nAChRs) not only increased susceptibility to stress by enhancing activity of VTA DA neurons, but also triggered a switch in phenotype from resilient to susceptible. Our data unravel dysregulations of VTA DA neurons activity exclusively in females exhibiting depressive-like symptoms and identify VTA nAChRs as key molecular substrates that exacerbate susceptibility to stress.

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