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Comparative analysis of gene expression changes during differentiation in the Drosophila wing

ABSTRACT: Investigation of whole genome gene expression level changes in dissected Drosophila wings of the genotype w1118, at the wandering L3 larval stage, 2h, 6h, 18h, 24h and 36h After pupa formation. Stages after pupa formation were compared to the larval L3 stage to identify changes in gene expression. The data included here is further discussed in O’Keefe, Thomas, Edgar and Buttitta (2012). Combinatorial control of temporal gene expression in the Drosophila wing by enhancers and core promoters Submitted to BMC Genomics

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE36015 | GEO | 2012/03/01

SECONDARY ACCESSION(S): PRJNA151721

REPOSITORIES: GEO

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Project description:Hox genes regionalize the animal body axis by modifying complex morphogenetic and differentiation processes during development. The transformation of wings into halteres by the Hox gene Ultrabithorax (Ubx) in Drosophila melanogaster presents an excellent model system to study the transcriptional networks that control such complex developmental programmes. We have employed an inducible misexpression system to switch on Ubx in the wing epithelium at successive larval, prepupal and pupal stages, and have used microarray expression profiling to identify the primary transcriptional responses to Ubx. We find that Ubx regulates hundreds of downstream genes, mostly in a subtle manner. These targets are largely distinct at the different stages of appendage development and diversification. We have generated an experimental fly line combining the nabGal4NP3537-driver, a tub-GAL80ts transgene, and a UAS-UbxIa transgene (the control line was carrying a UAS-eGFP transgene instead). Our core microarray analysis has involved comparison of the transcriptional profile of experimental wings carrying the UAS-UbxIa transgene with that of control wings carrying the UAS-eGFP transgene. Pairwise comparisons have been carried out at three successive developmental stages, in particular at (i) the third instar larval wandering stage, about 4hrs before puparium formation at 29˚C, (ii) the prepupal stage, 6hrs after puparium formation (APF) at 29˚C, and (iii) the early pupal stage, 16hrs APF at 29˚C. Moreover, pairwise comparisons have been carried out with samples developed exclusively at 19˚C (UbxIa or eGFP expression OFF), as well as with samples collected at 16hrs after the temperature shift from 19 to 29˚C (UbxIa or eGFP expression ON). This has allowed us to distinguish the Ubx-dependent effects from the intrinsic expression differences between the fly lines used, and from the temperature-induced responses. We have carried out 4 biological replicates for each condition making a total of 48 hybridizations to Affymetrix Drosophila Genome 2.0 arrays.

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Comparative analysis of gene expression changes during differentiation in the Drosophila wing

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