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Diurnal and circadian gene expression profile of leaf throughout the entire growth of rice in the field

ABSTRACT: A continuous gene expression profiling of leaves was performed at regular interval during a 48-hr period in 9 different growth stages from transplanting until harvesting to characterize the diurnal and circadian gene expression profile of rice and to understand the transcriptional program and physiological state throughout entire the growth under natural field conditions.

ORGANISM(S): Oryza sativa Oryza sativa Japonica Group

PROVIDER: GSE36040 | GEO | 2012/12/03

SECONDARY ACCESSION(S): PRJNA151763

REPOSITORIES: GEO

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Diurnal and circadian gene expression profile of leaf throughout the entire growth of rice in the field

Project description:A continuous gene expression profiling of leaves was performed at regular interval during a 48-hr period in 9 different growth stages from transplanting until harvesting to characterize the diurnal and circadian gene expression profile of rice and to understand the transcriptional program and physiological state throughout entire the growth under natural field conditions. The uppermost fully expanded leaf in the main stem was collected at 2-hr intervals during a 48-hr period at 9 different growth stages encompassing the vegetative, reproductive and ripening stages. All samples were obtained from rice plants grown in the field during the 2008 cultivation season.

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Project description:A continuous gene expression profiling of leaves at regular interval from transplanting until harvesting was performed to understand the changes of transcriptional program and physiological state throughout entire growth of the rice plant. For continuous gene expression profile of leaf, the uppermost fully-expanded leaf, 1st leaf before 83 days after transplanting (DAT) and flag leaf from then on, in the main stem was sampled at 12:00 PM at regular intervals of 7 days encompassing 17 different growth stages with three replicates.

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Continuous gene expression profile of leaf at nighttime throughout the entire growth of rice in the field

Project description:A continuous gene expression profiling of leaves was performed during nighttime at regular interval from transplanting until harvesting to understand the changes of transcriptional program and physiological state throughout entire growth of the rice plant under natural field conditions. The uppermost fully-expanded leaf in the main stem corresponding to the 1st leaf until 83 days after transplanting (DAT) and the flag leaf from them on were sampled in two replicates at 12:00 midnight at 7- day intervals encompassing 17 different growth stages. All samples were obtained from rice plants grown in the field during the 2008 cultivation season.

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Continuous gene expression profile of leaf throughout the entire growth of rice in 2010

Project description:A continuous gene expression profiling of leaves at regular interval from transplanting until harvesting was performed to understand the changes of transcriptional program and physiological state throughout entire growth of the rice plant in 2010. For continuous gene expression profile of leaf, the uppermost fully-expanded leaf in the main stem was sampled at 12:00 PM at regular intervals of 7 days encompassing 17 different growth stages with three replicates during a normal rice growing season in 2010.

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