Genomics

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Transcriptional profiling of turkey muscle satellite cells transfected with versican-siRNA during proliferation and differentiation


ABSTRACT: In this study, we directly compared turkey muscle satellite cell gene expression between satellite cells with the gene versican (VCAN) knocked down by siRNA transfection and those transfected with a lipofectamine control using our 6K Turkey Skeletal Muscle Long Oligo (TSKMLO) microarray (GPL9788). The VCAN gene was previously identified as differentially expressed by genetic line and during development of turkey skeletal muscle (Sporer et al., 2011). Gene expression changes were investigated in satellite cells after 72 h of proliferation and after 48 h of differentiation. We identified novel candidate genes and pathways as playing potentially crucial roles in the VCAN-mediated effects on the normal processes of proliferation and differentiation in turkey satellite cells previously identified by Velleman et al. (submitted).

ORGANISM(S): Meleagris gallopavo

PROVIDER: GSE36365 | GEO | 2012/06/01

SECONDARY ACCESSION(S): PRJNA153335

REPOSITORIES: GEO

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