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Expression data MCF10A/ErbB2 RB-proficient and RB-deficient cells cultured in 3-D cell culture

ABSTRACT: Gene expression analysis for structural components in Rb-deficient and RB-proficient ErbB2-overexpressing immortalized breast cell line using 3-D cell culture model to analyze structural and organizational changes of epithelial cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE36425 | GEO | 2013/03/01

SECONDARY ACCESSION(S): PRJNA153227

REPOSITORIES: GEO

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Expression data MCF10A/ErbB2 RB-proficient and RB-deficient cells cultured in 3-D cell culture

Project description:Gene expression analysis for structural components in Rb-deficient and RB-proficient ErbB2-overexpressing immortalized breast cell line using 3-D cell culture model to analyze structural and organizational changes of epithelial cells. Cells were cultured in a reconstituted membrane for 7 days. Cells were extracted with the use RNA-STAT-60 method.

2013-03-01 | E-GEOD-36425 | biostudies-arrayexpress

Targeting the vulnerability of RB tumor suppressor loss in triple negative breast cancer

Project description:Approximately 30% of TNBCs exhibit loss of function of the RB tumor suppressor. The study used RNA sequencing to profile RB-proficient and RB-deficient TNBC cases that were defined based on immunostaining for RB and p16ink4a. The analyses revealed that RB-deficient TNBC cases express elevated levels of DNA replication and mitotic genes that could serve as the basis for increased sensitivity to drugs targeting cell cycle checkpoints.

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Gene expression profiling of ErbB2-engineered MCF10A and WT cells

Project description:Gene expression profiling of ErbB2-engineered MCF10A and WT cells in 2D and 3D culture Gene expression profiling of ErbB2-engineered MCF10A and WT cells, 2D and 3D culture, 5 or 6 replicates

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Understanding BRCA1 function in INK4-RB deficient tumors

Project description:Most BRCA1-deficient BLBCs carry a dysfunctional INK4-RB pathway. Thus, we have created genetically engineered mice with Brca1 loss and deletion of p16INK4A, or separately p18INK4C, to model the deficient INK4-RB signaling in human BLBC. By using these mutant mice and human BRCA1 deficient and proficient breast cancer tissues and cells, we tested if there exists a druggable target in BRCA1 deficient breast cancers.

2021-01-27 | GSE155239 | GEO

Proteomic and phosphoproteomic profiling of ErbB2-dependent signalling

Project description:The mechanisms of ErbB2 signalling and the effects of its overexpression are not fully understood. Herein, SILAC expression profiling and phosphopeptide enrichment of a relevant, non-transformed, immortalized human mammary luminal epithelial cell model were used to profile ErbB2-dependent differences in protein expression and phosphorylation events triggered via EGFR (EGF treatment) and ErbB3 (HRGbeta1 treatment) in the context of ErbB2 overexpression. Bioinformatics analysis was used to infer changes in cellular processes and signalling events. We demonstrate the complexity of the responses to oncogene expression and growth factor signalling and identify protein changes relevant to ErbB2-dependent altered cellular phenotype, in particular cell cycle progression and hyper-proliferation, reduced adhesion and enhanced motility. Numerous novel sites of growth factor-regulated phosphorylation were also identified that were enhanced by ErbB2 overexpression and we putatively link these to altered cell behaviour. Moreover, we have defined a novel mechanism by which ErbB signalling suppresses basal interferon signalling that would promote the survival and proliferation of mammary luminal epithelial cells.

2018-10-26 | PXD004195 | Pride

Expression data from ERBB2 over-expression and EGF stimulation in MCF10A cells

Project description:Expression data from ERBB2 over-expression and EGF stimulation in MCF10A cells

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Expression data from ERBB2 over-expression and EGF stimulation in MCF10A cells

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2010-02-21 | E-GEOD-14987 | biostudies-arrayexpress

CSE1L and NUP37 proximity labeling in MCF10A cells expressing chimeric ErbB1:ErbB2 receptors

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2023-03-22 | PXD038327 | Pride

Hi-C of fibroblasts and germ cells from wild mice with Rb fusions

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