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SCFA receptor response

ABSTRACT: The purpose of this study was to determine the gene expression patterns of the colon of GPR41 KO and GPR43 KO mice in response to ETOH treatment

ORGANISM(S): Mus musculus

PROVIDER: GSE36569 | GEO | 2013/08/25

SECONDARY ACCESSION(S): PRJNA158633

REPOSITORIES: GEO

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SCFA receptor response

Project description:The purpose of this study was to determine the gene expression patterns of the colon of GPR41 KO and GPR43 KO mice in response to ETOH treatment The mice were treated with rectal injection of ETOH. 24 hours later, the colon tissues were harvested and total RNA was isolated for array experiments.

2013-08-25 | E-GEOD-36569 | biostudies-arrayexpress

Retinoic Acid Receptor β Loss in Hepatocytes Increases Steatosis and Elevates the Integrated Stress Response in Alcohol-Associated Liver Disease

Project description:Background & Aims: Alcohol-associated liver disease (ALD) is associated with loss of vitamin A (retinoids), increased steatosis, and greater oxidative stress. Although a strong correlation has been observed between loss of retinoids and ALD, if retinoid loss plays a causal role in the pathophysiology of ALD is not clear. Approach & Results: Based on our prior published data indicating that a selective retinoic acid receptor beta (RARβ) agonist limits nonalcohol-related liver disease (NAFLD) pathology, we generated AlbCre;RARβ knockout (BKO) mice and fed these mice a Lieber DeCarli ethanol diet (ETOH). We also generated cultured human hepatocytes (HepG2) that lack RARβ (RARβ-KO) and treated them with ETOH. Here we show that the livers of BKO mice develop greater steatosis, oxidative stress (assessed by 4-HNE), cell proliferation, and hypertrophy on the ETOH diet when compared to wild type (WT). Compared to ETOH-WT mice, the ETOH-BKO mice also exhibit higher hepatic transcript levels of ATF4, MYC, a subset of integrated stress response (ISR) genes (Trib3, Asns, Nqo1, and Hmox1), and the common ATF4 and MYC target, 4EBP1(EIF4EBP1). In RARβ-KO HepG2 cells, ETOH treatments (24 and 72h) resulted in more rapid and greater development of reactive oxygen species compared to parental HepG2. Notably, even without ETOH, ATF4 protein was higher in the RARβ-KO than in parental HepG2. Conclusions: Our research identifies hepatocyte RARβ as a crucial negative regulator of oxidative stress, MYC, ATF4, and proliferation in ALD.

2023-11-28 | GSE221713 | GEO

Transcription profiling by array of human DA-MB-231 breast cancer cells treated with various SCFA-hexosamine analogs

Project description:The purpose of this study was to characterize the gene expression profile of MDA-MB-231 breast cancer cells treated with various SCFA-hexosamine analogs to better understand the role of various modifications to this scaffold. Experiment Overall Design: In total, six analogs were investigated in this study. For the relatively ""toxic"" C6-SCFA analogs, IC30 was selected to explore initial growth inhibitory and apoptotic gene expression changes. Two independent biological replicates were analyzed for each analog-treated condition. Three independent biological replicates were analyzed for the vehicle-treated control (EtOH).

2009-05-17 | E-GEOD-11407 | biostudies-arrayexpress

SCFA-hexosamine scaffold

2009-05-08 | GSE11407 | GEO

WT vs IFN-gamma treated bulk RNA-seq libraries of HSCs

Project description:Purpose: to determine changes in RNA expression in WT vs BST2 KO mice following 24 hour treatment of IFNg. We were particularly interested in performing pathway analysis using GSEA.

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Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [ILC2_after_irr]

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Pregnane X receptor promotes ehtanol-induced hepatosteatosis in female mice

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2022-11-15 | PXD031487 | Pride

Global proteome of Atg16L1flox:CAG-CreTmx WT and KO neurons

Project description:The experiment was performed to identify autophagy targets in wildtype and autophagy-deficient primary neurons. Therefore, cortico-hippocampal neurons were isolated from Atg16L1flox:CAG-CreTmx mice and treated with Tamoxifen (Tmx) to induce the knockout (KO) or EtOH for wildtype (WT) in-vitro. WT and KO Neurons were harvested at day in-vitro (DIV) 15-16, and global proteome analysis was measured by LC-MS/MS.

2022-11-15 | PXD031555 | Pride

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