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Wt dex induction-Establishing the network of seed gene expression and analysis of its biodiversity

ABSTRACT: tri33-lec1 - wt dex induction - Identification of LEC1 target genes - Induction of 14 days old WT seedlings , different treatments ( EtOH, DEX induction) for 8 hours

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE36628 | GEO | 2012/03/27

SECONDARY ACCESSION(S): PRJNA156931

REPOSITORIES: GEO

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Wild type Lec1 transgene induction by DEX: Establishing the network of seed gene expression and analysis of its biodiversity (wt dex induction)

Project description:wt dex induction - Identification of LEC1 target genes - Induction of 14 days old WT seedlings , different treatments ( EtOH, DEX induction) for 8 hours

2011-08-31 | GSE22059 | GEO

wt dex induction-Establishing the network of seed gene expression and analysis of its biodiversity

Project description:tri33-lec1 - wt dex induction - Identification of LEC1 target genes - Induction of 14 days old WT seedlings , different treatments ( EtOH, DEX induction) for 8 hours 2 dye-swap - treated vs untreated comparison

2012-03-26 | E-GEOD-36628 | biostudies-arrayexpress

Wild type Lec1 transgene induction by DEX: Establishing the network of seed gene expression and analysis of its biodiversity (wt dex induction)

Project description:wt dex induction - Identification of LEC1 target genes - Induction of 14 days old WT seedlings , different treatments ( EtOH, DEX induction) for 8 hours 2 dye-swap - CATMA arrays

2011-08-30 | E-GEOD-22059 | biostudies-arrayexpress

LEC1::GR induction: Establishing the network of seed gene expression and analysis of its biodiversity

Project description:lec1::gr induction - Identification of LEC1 target genes - Induction of 14 days old LEC1::GR seedlings , different treatments (EtOH, DEX, DEX+ABA, induction) for 8 hours

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LEC1::GR induction: Establishing the network of seed gene expression and analysis of its biodiversity

Project description:lec1::gr induction - Identification of LEC1 target genes - Induction of 14 days old LEC1::GR seedlings , different treatments (EtOH, DEX, DEX+ABA, induction) for 8 hours 6 dye-swap - CATMA arrays

2011-08-30 | E-GEOD-22173 | biostudies-arrayexpress

RNA-Seq in LPS and Dex+LPS treated bone marrow-derived macrophages

Project description:Wild-type bone marrow-derived macrophages (BMDMs) were treated with vehicle (0.1% EtOH/D-PBS), 6h 100 ng/ml lipopolysaccharide (LPS) or 16h 1uM dexamethasone (Dex) and 6h 100 ng/ml LPS (Dex+LPS) and mRNA expression analysed by RNA-Seq.

2020-12-31 | GSE137412 | GEO

Transcript profiling of plants ectopically expressing DOF5.4 / OBF BINDING PROTEIN 4 (OBP4).

Project description:Transcription profiling of seven-day-old seedlings of p35S:OBP4-GR treated with DEX or EtOH for 12 and 24 h. Purpose: find transcriptionally regulated genes downstream of the dof transcription factors OBP4 in the root tip of Arabidopsis thaliana Method: compare transcript levels after ectopic induction of OBP4 for 12 h and 24 h. Ectopic expressing was induced by introducing the coding sequence of OBP4 fused to the glucocorticoid receptor under the control of the Cauliflower Mosaic Virus p35S promoter (p35S:OBP4-GR) in wild type Arabidopsis Col-0 plants. Seven day old plants expressing the construct were treated with Dexamethason (DEX) or ethanol (EtOH) as a mock for 12 and 24 h. After root tips of 300 seedlings per rep were collected.

2017-02-23 | E-MTAB-4838 | biostudies-arrayexpress

Arabidopsis thaliana

Project description:Wild type Lec1 transgene induction by DEX: Establishing the network of seed gene expression and analysis of its biodiversity (wt dex induction)

| PRJNA128965 | ENA

mRNA changes following induction of LEC1 activity in Arabidopsis seedlings

Project description:LEAFY COTYLEDON1 (LEC1), an atypical subunit of the NF-Y CCAAT binding transcription factor, is a central regulator that controls many aspects of seed development including the maturation phase during which seeds accumulate storage macromolecules and embryos acquire the ability to withstand desiccation. To define the gene network and developmental processes controlled by LEC1, genes regulated directly by and downstream of LEC1 were identified. In this part of the study, we identified the genes regulated by LEC1 by overexpressing an inducible form of LEC1 in Arabidopsis seedlings and identifying the mRNAs induced EARLY (1h) after induction of LEC1 activity.

2017-07-12 | GSE99528 | GEO

Identification of LEC1 target genes

Project description:The transcription factor (TF) LEAFY COTYLEDON1 (LEC1) acts as an essential regulator of Arabidopsis embryogenesis and seed development. It controls aspects of early embryogenesis such as cotyledon identity and suspensor morphology, as well as seed maturation processes such as storage compound accumulation, acquisition of desiccation tolerance and dormancy. To identify downstream components of the LEC1 regulon, dexamethasone-regulated expression of LEC1 and ChIP/chip were applied and revealed the enrichment of phytohormone- as well as elongation-related genes among LEC1 target genes. Two weeks old 35S::LEC1::GR seedlings were treated with DEX or ethanol for 24 hours before cross-linking and chromatin isolation. Four biological replicates were performed, using an ethanol-treated sample as control (LEC1_EtOH versus LEC1_DEX). After data normalization, statistical analysis and exclusion of putative false negatives, a number of 356 target promoter regions were identified.

2012-03-30 | E-GEOD-22352 | biostudies-arrayexpress

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