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Genome-wide expression profiling in a Fusarium graminearum lacking FgVelB, a velvet complex gene

ABSTRACT: Using a 3'-tiling microarray covering the whole F. graminearum genes, we carried out genome-wide expression analyses of both a wild type and a FgVelB deletion strainn of F. graminearum at a sexual stage (3 days after perithecial induction on carrot agar) Our study is the first report elucidating regulatory pathways controlled by FgVelB in Fusarium graminearum.

ORGANISM(S): Fusarium graminearum

PROVIDER: GSE36638 | GEO | 2012/08/03

SECONDARY ACCESSION(S): PRJNA153639

REPOSITORIES: GEO

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Genome-wide expression profiling in a Fusarium graminearum lacking FgVelB, a velvet complex gene

Project description:Using a 3'-tiling microarray covering the whole F. graminearum genes, we carried out genome-wide expression analyses of both a wild type and a FgVelB deletion strainn of F. graminearum at a sexual stage (3 days after perithecial induction on carrot agar) Our study is the first report elucidating regulatory pathways controlled by FgVelB in Fusarium graminearum. A total of 4 chips were used for microarray. Total RNAs were extracted from perithecia and/or ground mycelia formed on carrot agar cultures of a self-fertile wild-type and a self-sterile FgVelB-deleted strain of F. graminearum with two biological replicates.

2012-08-02 | E-GEOD-36638 | biostudies-arrayexpress

Genome-wide expression profiling reveals transcriptional reprogramming in Fusarium graminearum by FgV1-DK21 virus infection

Project description:Using a 3′-tiling microarray covering the whole F. graminearum genes, we carried out genome-wide expression analyses of F. graminearum infected with FgV-DK21 (Mycovirus) at two different time stages (36 h and 120 h). Our study is the first report which elucidated the global alternation of the prominent cellular pathways in fungal host by FgV1-DK21 virus infection. A total of 12 chips were used for microarray. At 36 h and 120 h, total RNAs were extracted from virus free Fusarium and virus infected Fusarium with three biological replicates.

2012-03-01 | E-GEOD-30545 | biostudies-arrayexpress

Genome-wide expression profiling reveals putative target genes of the mating type loci (MAT1-1 and MAT1-2) in Fusarium graminearum during sexual devleopment

Project description:Using a 3′-tiling microarray covering the whole F. graminearum genome, we carried out genome-wide expression analyses of F. graminearum strains deleted for MAT1-1 or MAT1-2 locus, or overexpression the MAT1-2-1 gene during the sexual devleopment Our study is the first report which elucidated the putative target genes of the mating type genes in Fusarium graminearum during sexual development

2015-01-01 | GSE58543 | GEO

RNA-seq Analysis of FlbD-, AbaA-, and WetA-dependent Genes in Fusarium graminearum Z-3639

Project description:Fusarium graminearum (teleomorph Gibberella zeae) is a prominent pathogen that infects major cereal crops, such as wheat, barley, and maize. Conidiogenesis had been intensively studied in Aspergillus nidulans and regulatory pathway genes have been known to regulate conidiogenesis in stage specific manner. We reported the functional analyses of flbD, abaA, and wetA orthologs in F. graminearum. To understand genome-wide transcriptional profiling of conidiation, we employed RNA-seq of the wild-type Fusarium graminearum Z-3639 and each gene deletion mutants with three time courses (0 h, 6 h and 12 h after induction of conidiogenesis). AbaA experiment: 6 samples examined: 0 h, 6 h and 12 h after induction of conidiogenesis of Fusarium graminearum Z-3639 wild type and ΔabaA(ΔabaA::gen) mutant strains WetA experiment: 3 samples examined: 0 h, 6 h and 12 h after induction of conidiogenesis of Fusarium graminearum ΔwetA(ΔwetA::gen) mutant strains flbD experiment: 3 samples examined: 0 h, 6 h and 12 h after induction of conidiogenesis of Fusarium graminearum ΔflbD(ΔflbD::gen) mutant strains

2013-11-10 | E-GEOD-46133 | biostudies-arrayexpress

RNA-seq analysis of Fusarium graminearum Z-3639 in heat stress

Project description:Fusarium graminearum (teleomorph Gibberella zeae) is a prominent pathogen that infects major cereal crops, such as wheat, barley, and maize. To dissect cellular responses toward heat stress in the plant pathogenic fungus F. graminearum, we compared transcriptomes of the fungal cultures incubated in normal temperature condition (25 ÂºC) and in high temperature condition (37 ÂºC) for 15 min. 6 samples examined: 24 h-old mycelia from complete medium (CM) of Fusarium graminearum wild-type Z-3639 were incubated in normal temperature condition (25 ÂºC) and in high temperature condition (37 ÂºC) for 15 min.

2016-05-13 | E-GEOD-78885 | biostudies-arrayexpress

Fusarium graminearum

Project description:Fusarium graminearum Assembly

| PRJNA297826 | ENA

Transcriptional responses of Fusarium graminearum to plant cell wall substrates

Project description:We report a complete transcriptomic study of Fusarium graminearum in response to glucose, cellulose, xylan and cell wall fragments with a whole genome microarray from febit. Fusarium graminearum was cultured at 25 °C on minimal M3 medium with glucose, birch wood xylan, carboxy methyl cellulose or hop cell wall as sole carbon source at a concentration of 10 g/L. Microarray experiments were achieved with a Geniom device (febit biomed, Germany). Each of the four tested condition was tested with two biological and two technical replicates.

2011-06-16 | E-GEOD-29973 | biostudies-arrayexpress

Fusarium graminearum in vitro secretome

Project description:The plant pathogenic fungus Fusarium graminearum (Fgr) creates economic and health risks in cereals agriculture. Fgr causes head blight (or scab) of wheat and stalk rot of corn, reducing yield, degrading grain quality and polluting downstream food products with mycotoxins. Fungal plant pathogens must secrete proteases to access nutrition and to breakdown the structural protein component of the plant cell wall. Research into the proteolytic activity of Fgr is hindered by the complex nature of the suite of proteases secreted. We used a systems biology approach comprising genome analysis, transcriptomics and label-free quantitative proteomics to characterise the peptidases deployed by Fgr during growth. A combined analysis of published microarray transcriptome datasets revealed seven transcriptional groupings of peptidases based on in vitro growth, in planta growth, and sporulation behaviours. An orbitrap MS/MS proteomics technique defined the extracellular proteases secreted by Fusarium graminearum.

2015-10-06 | PXD002786 | Pride

RNA-seq analysis of FHS1-dependent genes in Fusarium graminearum Z-3639

Project description:Fusarium graminearum (teleomorph Gibberella zeae) is a prominent pathogen that infects major cereal crops, such as wheat, barley, and maize. Fhs1 contains a Zn(II)2Cys6 fungal-type DNA-binding domain and localized to nuclei , suggesting that Fhs1 is a transcription factor required for hydroxiurea. 6 samples examined: 24 h after inoculation of Fusarium graminearum wild-type Z-3639 and fhs1 (Îfhs1::GEN) strains in complete media

2016-02-22 | E-GEOD-73891 | biostudies-arrayexpress

Gene expression profile in MS medium for the phytopathogenic fungus Fusarium graminearum

Project description:Full transcriptome of the Fusarium graminearum wild-type strain CBS185.32, grown in MS medium, was studied.

2021-12-04 | GSE162839 | GEO