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Comparison_col0vsskp2-Functional analysis of the SKP2 and CUL4 proteins

ABSTRACT: rs04-04_skp2_cul4 - comparison_col0vsskp2 - Antisense and mutants of the SKP2 and CUL4 genes are compared to the Wt in order to identify the genes controlled by those SCF proteins. - Comparison Col0 versus skp2

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE36651 | GEO | 2012/03/27

SECONDARY ACCESSION(S): PRJNA156935

REPOSITORIES: GEO

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comparison_col0vsskp2-Functional analysis of the SKP2 and CUL4 proteins

Project description:rs04-04_skp2_cul4 - comparison_col0vsskp2 - Antisense and mutants of the SKP2 and CUL4 genes are compared to the Wt in order to identify the genes controlled by those SCF proteins. - Comparison Col0 versus skp2 2 dye-swap - wt vs mutant comparison

2012-03-26 | E-GEOD-36651 | biostudies-arrayexpress

comparison_cul4_as_light-Functional analysis of the SKP2 and CUL4 proteins

Project description:rs04-04_skp2_cul4 - comparison_cul4_as_light - Antisense and mutants of the SKP2 and CUL4 genes are compared to the Wt in order to identify the genes controlled by those SCF proteins. - The seeds have been sown every two days on MS 255 (Duschefa) without 1% sugar. These plates stayed 6 days in the dark at 4 Â°C. Then they were transfered to the light for 6 more days. 4 dye-swap - gene knock out, normal vs antisense mutant comparison

2012-03-26 | E-GEOD-36655 | biostudies-arrayexpress

comparison_cul4_as_dark_light-Functional analysis of the SKP2 and CUL4 proteins

Project description:rs04-04_skp2_cul4 - comparison_cul4_as_dark_light - Antisense and mutants of the SKP2 and CUL4 genes are compared to the Wt in order to identify the genes controlled by those SCF proteins. - The seeds have been sown every two days on MS 255 (Duschefa) without adding sugar. These plates stayed 2 days in the dark at 4Â°C. Then they got a 8 hours light induction (light induction of germination) and then back to the dark for 5 more days. 4 dye-swap - gene knock out,normal vs antisense mutant comparison

2012-03-26 | E-GEOD-36652 | biostudies-arrayexpress

comparison_cul4_as_light-Functional analysis of the SKP2 and CUL4 proteins

Project description:rs04-04_skp2_cul4 - comparison_cul4_as_light - Antisense and mutants of the SKP2 and CUL4 genes are compared to the Wt in order to identify the genes controlled by those SCF proteins. - The seeds have been sown every two days on MS 255 (Duschefa) without 1% sugar. These plates stayed 6 days in the dark at 4 °C. Then they were transfered to the light for 6 more days.

2012-03-27 | GSE36655 | GEO

comparison_cul4_as_dark_light-Functional analysis of the SKP2 and CUL4 proteins

Project description:rs04-04_skp2_cul4 - comparison_cul4_as_dark_light - Antisense and mutants of the SKP2 and CUL4 genes are compared to the Wt in order to identify the genes controlled by those SCF proteins. - The seeds have been sown every two days on MS 255 (Duschefa) without adding sugar. These plates stayed 2 days in the dark at 4°C. Then they got a 8 hours light induction (light induction of germination) and then back to the dark for 5 more days.

2012-03-27 | GSE36652 | GEO

The Replication-initiation determinant protein (RepID) modulates replication by recruiting CUL4 to chromatin

Project description:Cell cycle progression in mammals is modulated by two ubiquitin ligase complexes, CRL4 and SCF, which facilitate degradation of chromatin substrates involved in the regulation of DNA replication. One member of the CRL4 complex, RepID (DCAF14/PHIP), is a WD40-containing protein that selectively binds and activates a group of replication origins. We found that RepID recruits the CRL4 complex to chromatin prior to DNA synthesis, thus playing a crucial architectural role in the proper licensing of chromosomes for replication. In the absence of RepID, cells rely on the alternative ubiquitin ligase, SKP2-containing SCF, to progress through the cell cycle. RepID depletion markedly increases cellular sensitivity to SKP2 inhibitors, which triggered massive genome re-replication. Both RepID and SKP2 interact with distinct, non-overlapping groups of replication origins, suggesting that selective interactions of replication origins with specific CRL components execute the DNA replication program and maintain genomic stability by preventing re-initiation of DNA replication.

2018-06-27 | GSE114703 | GEO

MDAMB231 transcriptome response to SKP2 knockdown

Project description:The SKP2 was knockdown by siRNAs in MDA-MB231 cells and RNA-Seq was used to define the transcriptome response to SKP2

2020-12-14 | GSE163110 | GEO

nt-kis-CKI role in plant development

Project description:rs04-08_ntkis - nt-kis - Transcription profiling of NtKIS overexpressing lines - 35S NtKIS (CDK inhibitor) versus Col0 Keywords: gene knock in (transgenic)

2007-08-09 | GSE8697 | GEO

CKI role in plant development

Project description:rs04-08_ntkis - nt-kis - Transcription profiling of NtKIS overexpressing lines - 35S NtKIS (CDK inhibitor) versus Col0 Keywords: gene knock in (transgenic)

2007-05-31 | GSE7939 | GEO