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Apc transcriptome-Transcriptome on Arabidopsis APC hypomorphic lines

ABSTRACT: rs07-03_apc - apc transcriptome - Which genes are misregulated when the APC complex activity is reduced? - Wild Type Col0, APC10S and CDC16S lines (down regulation of APC10 and CDC16 endogenous genes due to overexpression of a sequence corresponding to a part of APC10 or CDC16 gene, respectively) are grown in growth chamber (16h photoperiod 22°C) for 15 days. Whole seedlings are then frozen in liquid nitrogen and total RNA are extracted.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE36653 | GEO | 2012/03/27

SECONDARY ACCESSION(S): PRJNA156939

REPOSITORIES: GEO

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apc transcriptome-Transcriptome on Arabidopsis APC hypomorphic lines

Project description:rs07-03_apc - apc transcriptome - Which genes are misregulated when the APC complex activity is reduced? - Wild Type Col0, APC10S and CDC16S lines (down regulation of APC10 and CDC16 endogenous genes due to overexpression of a sequence corresponding to a part of APC10 or CDC16 gene, respectively) are grown in growth chamber (16h photoperiod 22Â°C) for 15 days. Whole seedlings are then frozen in liquid nitrogen and total RNA are extracted. 4 dye-swap - normal vs rnai mutant comparison

2012-03-26 | E-GEOD-36653 | biostudies-arrayexpress

Cell cycle and cell death interaction in plants: PCNA, AtXRs and AtMIPS pathway.

Project description:rs07-04_mips - atmips mutant/photoperiod - In Arabidopsis thaliana, how does MIPS knock out influence gene expression? In AtMIPS mutants how does photoperiod influence gene expression. - Wild type Colombia and MIPS mutant (Salk 023626) plants are grown in short days (8h light) during 27 days and then transfered in long days (16h light) during 4 days. J4JC correspond to 27+4=31 days plants in short days. Keywords: gene knock out

2009-03-07 | GSE15123 | GEO

Cell cycle and cell death interaction in plants: PCNA, AtXRs and AtMIPS pathway.

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Interactions between H2O2 and glutathione in gene expression

Project description:rs08-03_glutathion - glutathion - How does glutathione content or reduction state affect H2O2-induced changes in the transcriptome? - Three single Arabidopsis mutants were used: cat2, knockout for catalase2 and so enriched in H2O2; cad2, defective in glutathione content; cytGR, knockout for cytosolic glutathione reductase. Cat2 was crossed with cad2 and cyt GR, and col0, 3 single mutants, and 2 double mutants were sampled in controlled growth conditions either in 8h or 16h photoperiod. Keywords: gene knock out

2010-07-22 | GSE21424 | GEO

Photooxidative stress and light acclimation in the PSII antenna-deficient ch1 mutant

Project description:cea10-02_light - photooxidative stress - To characterize the metabolic pathways implicated in oxidative stress responses and in acclimation mechanisms in the ch1 mutant - The extracts are carried out starting from sheets of Arabidopis thaliana having pushed on compost in controlled conditions (light: 250 µmol.m-2.s-1, temperature: 22°C day 18°C night, moisture: 55%, photoperiod: 8:00 jour/16h night) during 4 and 8 weeks for respectively the Col0 genotype and the mutant ch1. The acclimatization of the plants is done during 48 H under an average luminous intensity (450 µmol.m-2.s-1, photoperiod 8:00), whereas the stress requires a strong luminous intensity (900 µmol.m-2.s-1, photoperiod 8:00) and low temperature (10°C day, 14°C night) also during 48 hours.

2011-12-09 | GSE34247 | GEO

Interactions between H2O2 and glutathione in gene expression

2010-07-22 | E-GEOD-21424 | biostudies-arrayexpress

Photooxidative stress and light acclimation in the PSII antenna-deficient ch1 mutant

2011-12-09 | E-GEOD-34247 | biostudies-arrayexpress

Metabolomics of Diapause in Aedes albopictus

Project description:Replicate populations of Aedes albopictus were reared under diapause-inducing short day photoperiod (8h light: 16h dark) and diapause-averting long day photoperiod (16h light:8h dark). Eggs were collected from each replicate and snap-frozen 11d post-oviposition. We hope to characterize the metabolomic and lipidomic profiles of diapausing eggs relative to non-diapause eggs.

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Apc and ApcKras

Project description:Apc and ApcKras

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myb52-2 t-dna -Transcriptome analysis of atmyb52 mutant vs wild-type

Project description:rs11-11_myb52 - myb52-2 t-dna - What are the structural and regulator genes regulated by MYB52 transcription factor? - Plants grew in short-day growth chamber until they reached 20 cm height. Ten centimeter-stem from the rosette were collected. Samples are pools of 8 wild-type (Col0) or (atmyb52) mutant plants. Two biological repeats were conducted.

2013-02-01 | GSE43647 | GEO

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