Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Protease Treatment

ABSTRACT: Determine if transcripts are released by protease treatment Keywords: Comparison of protease treatments

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE3686 | GEO | 2005/12/01

SECONDARY ACCESSION(S): PRJNA93829

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Protease Treatment

Project description:Determine if transcripts are released by protease treatment During total RNA isolation cell free lysates from stationary-phase cultures or exponential cultures were treated with one of three proteases or buffer alone. All experimental samples are over a common reference. There are two replicates for each sample.

2010-06-30 | E-GEOD-3686 | biostudies-arrayexpress

Enzymatic treatment using trypsin and cold-active protease for C2C12 myoblasts

Project description:We have comprehensively profiled the time-series changes in gene expression dependent on enzymatic treatment using trypsin, and attempted to elucidate the global changes suppressed by treatment with cold active protease in a low-temperature environment.

2022-06-20 | GSE168452 | GEO

Heat-treatments affect protease activities and peptide profiles of ruminants’ milk

Project description:Proteases present in milk are heat-sensitive, and their activities increase or decrease depending on the intensity of the thermal treatment applied. The thermal effects on the protease activity are well-known for bovine milk but poorly understood for ovine and caprine milk. This study aimed to determine the nonspecific and specific protease activities in casein and whey fractions isolated from raw bovine, ovine and caprine milk collected in early lactation, and to determine the effects of low-temperature, long-time (63°C for 30 min) and high-temperature, short-time (85°C for 5 min) treatments on protease activities within each milk fraction.

2021-09-09 | PXD022702 | Pride

microRNA profile of control vehicle- and coagulation protease factor VIIa-released endothelial extracellular vesicles

Project description:Coagulation protease factor VIIa (FVIIa) is shown to induce anti-inflammatory and barrier protective effects via endothelial cell protein C receptor (EPCR)-dependent, protease-activated receptor-1 (PAR1)-mediated cell signaling. FVII-EPCR-PAR1 signaling also induces the release of extracellular vesicles from endothelial cells. To obtain clues on whether microRNA (miR) carried out by FVIIa-released EEVs contribute to anti-inflammatory and barrier protective effects, we analyzed miR expression in control- and FVIIa-released EEVs by deep sequencing. These data revealed that several anti-inflammatory miR expression was higher (more than 2-fold) in FVIIa-released EEVs compared to control EEVs, the most predominant being miR10a-5p. The differential expression of miR10a-5p and several other abundant miRs were validated by qRT-PCR. Subsequent in vitro and in vivo experiments showed that miR10a in FVIIa-released EEVs contribute to anti-inflammatory and barrier protective effects.

2021-08-29 | GSE182925 | GEO

Protease-activated receptor-2 promotes osteogenesis in skeletal mesenchymal stem cells at the expense of adipogenesis

Project description:Osteoblast-like cells isolated from the calvariae of wildtyope and protease-activated receptor-2 null were treated with unconditioned medium (Control) or medium conditionedby the prostate cancer cell line MDA-PCa-2b (MDA). RNAseq analysis was performed to identify differntially gene expression in wildtype and protease-activated receptor-2 null in response to factors released by prostate cancer cells.

2019-08-16 | GSE135865 | GEO

Polytopic protease downregulation effect on the brain (rogae-affy-arabi-492460)

Project description:Regulation of AD-related genes. Presenilins are intramembrane polytopic proteases. These proteases are critical proteins in pathogenesis of Alzheimer's disease. The function of other polytopic proteases expressed in brains is unknown. Proteins that may potentially interact with PS pathway are of interest to elucidate. In this project we will determine gene expression patterns in the brains of mice with altered expression of polytopic protease. We hypothesize that family of polytopic proteases expressed in brain may potentially interact with presenilin pathway. We will examine 4 RNA samples isolated from brains of mice with significant downregulation of polytopic protease and control animals. Total RNA was extracted, purified according to the manufacturer's protocol and stored at -80C. Keywords: polytopic protease, brain

2008-04-12 | GSE11148 | GEO

C/EBPa Regulates Protease/anti-protease Balance and Mediates Bronchiolar Cell Recovery After Injury

Project description:In the present study, we hypothesized that C/EBPa (CCAAT/enhancer-binding protein alpha) plays a role in cell regeneration in response to bronchiolar epithelial cell injury. C/EBPa mediated ciliated cell regeneration after naphthalene bronchiolar epithelial cell injury in vivo. Furthermore, we demonstrated that C/EBPa regulates protease/anti-protease balance after lung injury, and intratracheal treatment with anti-protease (BPTI) restored ciliated cell regeneration after naphthalene injury in CebpaD/D mice.

2012-05-13 | GSE29285 | GEO

C/EBPa Regulates Protease/anti-protease Balance and Mediates Bronchiolar Cell Recovery After Injury

2012-05-12 | E-GEOD-29285 | biostudies-arrayexpress

Enzymatic treatment using trypsin and cold-active protease for C2C12 myoblasts

Project description:Enzymatic treatment using trypsin and cold-active protease for C2C12 myoblasts

| PRJNA707443 | ENA

Protease Encoding bacteria

Project description:Protease Encoding bacteria in arable fields

| PRJEB24038 | ENA