Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparison of Bacillus subtilis wild type and cshA mutant at exponential versus stationary phase

ABSTRACT: Comparison of Bacillus subtilis wild type and cshA mutant at exponential versus stationary phase. Detailed description (other than provided below) of growth conditions, RNA preparation, cDNA synthesis and hybridization conditions can be found in the submitted paper.

ORGANISM(S): Bacillus subtilis

PROVIDER: GSE36877 | GEO | 2012/11/26

SECONDARY ACCESSION(S): PRJNA157001

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Comparison of Bacillus subtilis wild type and cshA mutant at exponential versus stationary phase

Project description:Comparison of Bacillus subtilis wild type and cshA mutant at exponential versus stationary phase. Detailed description (other than provided below) of growth conditions, RNA preparation, cDNA synthesis and hybridization conditions can be found in the submitted paper. Bacillus subtilis 168 wild type and its cshA derivative strain were grown in CSE-Glu medium and cells were harvested at mid exponential (OD600 ~0.8) and early stationary phase (OD600 ~2.2-2.4). For both time points, 4 biological replicates were used and 2 were mixed after cDNA labeling, resulting 2 slides for both exponential and stationary phase. Dye swaps are included in both experiments.

2012-11-26 | E-GEOD-36877 | biostudies-arrayexpress

The YmdB phosphodiesterase is a global regulator of biofilm-specific mRNA levels in Bacillus subtilis

Project description:Detailed description (other than provided below) of growth conditions, RNA preparation, cDNA synthesis and hybridization conditions can be found in the submitted paper. GSM956794-GSM956801: Bacillus subtilis 168 wild type and its ymdB derivative strain were grown in CSE-Glu medium and cells were harvested at mid exponential (OD600 ~0.8) and early stationary phase (OD600 ~2.2-2.4). For both time points, 4 biological replicates were used, resulting 4 slides for both exponential and stationary phase. Dye swaps are included in both experiments. GSM1011025-GSM1011032: Bacillus subtilis GP966 antibiotic tagged synthetic wild type and its ymdB E39Q point mutant strain GP969 were grown in CSE-Glu medium and cells were harvested at mid exponential (OD600 ~0.8) and early stationer phase (OD600 ~2.2-2.4). For both time points, 4 biological replicates were used, resulting 4 slides for both exponential and stationer phase. Dye swaps are included in both experiments.

2013-12-11 | E-GEOD-39142 | biostudies-arrayexpress

Bacillus subtilis WalR (YycF) ChIP-chip Bacillus subtilis; Bacillus subtilis subsp. subtilis str. 168

2015-01-26 | GSE65272 | GEO

Attachment of Bacillus subtilis to the hyphae of Aspergillus niger results in altered metabolism and defense mechanisms in both partner

Project description:Transcriptome comparison of Bacillus subtilis NCIB3610 attached to the hyphae of Aspergillus niger CB 119.1 compared to Bacillus subtilis NCIB3610 cells in the supernatant of the same culture. Detailed description (other than provided below) of growth conditions, RNA preparation, cDNA synthesis and hybridization conditions can also be found in the submitted paper.

2014-09-09 | GSE46682 | GEO

Bacillus subtilis SigA ChIP-chip (BsubT2 array)

Project description:Identification of the specific SigA binding regions on the B. subtilis chromosome during exponential, transition and stationary growth phases. The data served to help the analysis of the repertoire of B. subtilis promoters established from transcriptome profiles.

2012-01-01 | GSE27665 | GEO

Bacillus subtilis SigA ChIP-chip (BsubT1 array)

2012-01-01 | GSE27650 | GEO

Bacillus subtilis SigA ChIP-chip (BsubT2 array)

2012-01-01 | E-GEOD-27665 | biostudies-arrayexpress

Bacillus subtilis SigA ChIP-chip (BsubT1 array)

2012-01-01 | E-GEOD-27650 | biostudies-arrayexpress

Bacillus subtilis WN715 vs WN716 expression in early stationary phase.

Project description:Five batch cultures of Bacillus subtilis were subjected to laboratory evolution for 6,000 generations under conditions repressing sporulation in complex liquid medium containing glucose. Between 1,000 and 2,000 generations, variants with a distinct small-colony morphology arose and swept through four of the five populations. To understand the nature of adaptation in these variants, individual strains were isolated from one population before (WN715) and after (WN716) the sweep. In addition to colony morphology, strains WN715 and WN716 differed in their motility, aerotaxis, and cell morphology. Competition experiments of WN715 vs. WN716 showed that WN716 had gained a distinct fitness advantage during growth and the transition to stationary phase, which was more pronounced when WN715 was present in co-culture. Microarray analyses revealed candidate genes in which mutations may have produced some of the observed phenotypes.

2010-09-09 | GSE24018 | GEO

Bacillus subtilis WalR (YycF) ChIP-chip Bacillus subtilis; Bacillus subtilis subsp. subtilis str. 168

Project description:Identification of the specific WalR (YycF) binding regions on the B. subtilis chromosome during exponential and phosphate starvation growth phases. The data serves to extend the WalRK regulon in Bacillus subtilis and its role in cell wall metabolism, as well as implying a role in several other cellular processes. For each sample analyzed in this study three biological replicates were performed. Three different samples were taken from a strain expressing the WalR-SPA protein as well as from wild-type (168) without a tagged WalR. Samples were taken from exponentially growing cells in low phosphate medium (LPDM) as well as from phosphate-limited cells (T2). Each sample compares ChIP DNA vs. Total DNA from the same cells.

2015-01-26 | E-GEOD-65272 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data