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Global Transcriptome Analysis of Tropheryma whipplei in Response to Temperature Stresses

ABSTRACT: Microarray analysis of the global gene expression patterns of Tropheryma whipplei in response to thermal stresses showed unique transcription profiles. A few genes were regulated after 15 min exposure at 43°C, including the dnaK regulon composed of grpE, hspR, dnaK, clpB, cbpA as well as the TWT745 ORFan gene which might encode for another heat-shock protein. This operon is likely to be regulated by two HspR associated inverted repeats (HAIR motifs) found within its 5' region. Putative virulence factors like RibC and IspDF proteins were also up-regulated under such conditions. In contrast to the heat-shock response, T. whipplei transcriptome was strongly modified following cold shock at 4°C. From the 149 genes differentially transcribed, 9 regulons were evidenced, one of them being composed of 5 genes sharing similarities with ABC transporters. Up-regulation of the latter suggests an increase of nutrient uptake during cold stress. As evoked for several bacteria species, the major classes of differentially transcribed genes encode membrane proteins and enzymes involved in fatty acid biosynthesis, indicating that membrane modifications are essential. A most specific feature observed following T. whipplei in response to cold was the up-regulation of heat-shock proteins namely GroEL2 and ClpP1, as well as several genes involved in energy metabolism. All together, these data show that T. whipplei exhibits a specific adaptative response to thermic stresses which fits well to its putative environmental origin. Keywords: thermal stresses responses

ORGANISM(S): Tropheryma whipplei

PROVIDER: GSE3693 | GEO | 2005/12/02

SECONDARY ACCESSION(S): PRJNA93841

REPOSITORIES: GEO

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Project description:Voit2003 - Trehalose Cycle This model is described in the article: Biochemical and genomic regulation of the trehalose cycle in yeast: review of observations and canonical model analysis. Voit EO. J. Theor. Biol. 2003 Jul; 223(1): 55-78 Abstract: The physiological hallmark of heat-shock response in yeast is a rapid, enormous increase in the concentration of trehalose. Normally found in growing yeast cells and other organisms only as traces, trehalose becomes a crucial protector of proteins and membranes against a variety of stresses, including heat, cold, starvation, desiccation, osmotic or oxidative stress, and exposure to toxicants. Trehalose is produced from glucose 6-phosphate and uridine diphosphate glucose in a two-step process, and recycled to glucose by trehalases. Even though the trehalose cycle consists of only a few metabolites and enzymatic steps, its regulatory structure and operation are surprisingly complex. The article begins with a review of experimental observations on the regulation of the trehalose cycle in yeast and proposes a canonical model for its analysis. The first part of this analysis demonstrates the benefits of the various regulatory features by means of controlled comparisons with models of otherwise equivalent pathways lacking these features. The second part elucidates the significance of the expression pattern of the trehalose cycle genes in response to heat shock. Interestingly, the genes contributing to trehalose formation are up-regulated to very different degrees, and even the trehalose degrading trehalases show drastically increased activity during heat-shock response. Again using the method of controlled comparisons, the model provides rationale for the observed pattern of gene expression and reveals benefits of the counterintuitive trehalase up-regulation. To induce a heat shock, set the parameter heat_shock from 0 to 1. This changes the parameter values of X8 to X19 from 1 to the values given in table 3 of the original publication. As this is an S-systems model, it does not contain any reactions encoded in SBML. This model is hosted on BioModels Database and identified by: BIOMD0000000266. To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

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Global Transcriptome Analysis of Tropheryma whipplei in Response to Temperature Stresses

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