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Gene expression signatures of MEF2D-silencing hepatocellular carcinoma cells

ABSTRACT: Global expression profiles in Huh7 after infection with Lv-shMEF2D and Lv-scrambled, as well as transfection with siRNA-MEF2D and siRNA-control, were compared to investigate the molecular mechanisms involved in MEF2D-mediated regulation of cell cycle.

ORGANISM(S): Homo sapiens

PROVIDER: GSE37123 | GEO | 2015/04/07

SECONDARY ACCESSION(S): PRJNA158281

REPOSITORIES: GEO

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Gene expression signatures of MEF2D-silencing hepatocellular carcinoma cells

Project description:Global expression profiles in Huh7 after infection with Lv-shMEF2D and Lv-scrambled, as well as transfection with siRNA-MEF2D and siRNA-control, were compared to investigate the molecular mechanisms involved in MEF2D-mediated regulation of cell cycle. In order to investigate the molecular mechanisms involved in MEF2D-mediated regulation of cell cycle, we assessed gene expression profiles in Huh7 cells after infection with Lv-shMEF2D and Lv-scrambled, as well as transfection with siRNA-MEF2D and siRNA-control, by cDNA microarray. Analysis of global mRNA expression profile indicated a shift toward G2/M arrest in the cells after downregulating MEF2D expression. The genes that inhibit G2/M transition were found to be expressed in high level in MEF2D-downregulated group, as compared with control group. Meanwhile, mRNA abundance of G2/M transition-promoting genes, except CDC2 and CDC25C, was reduced when MEF2D expression was depressed in Huh7 cells

2015-04-07 | E-GEOD-37123 | biostudies-arrayexpress

RNA-seq for gene expression changes upon silencing MEF2D-fusion in a MEF2D-HNRNPUL1 fusion-expressing Kasumi-7 cell line

Project description:To explore the mechanisms underlying the maintenance of acute lymphoblastic leukemia harboring fusion genes involving MEF2D transcription factor, the MEF2D-fusion was silenced by shRNA and the resulting gene expression changes were analyzed by RNA-seq.

2020-06-30 | E-MTAB-8480 | biostudies-arrayexpress

Effects of knockdown of Jmjd6 on human umbilical vein endothelial cells - gene and exon expression

Project description:Human umbilical vein endothelial cells (HUVECs) were incubated for 48 h after transfection of scrambled siRNA or siRNA targeting Jmjd6 . Changes in transcript and exon levels were analyzed.

2012-05-04 | GSE37741 | GEO

Dysregulation of MITF in the context of defective MC1R and RB1/p16/CDK4 leads to melanocyte transformation [RNA-Seq]

Project description:RNAseq Expression analysis of immortalized melanocytes Hermes 3c (3C) and Hermes 4c (4C) cell lines following siRNA transfection with a control scrambled siRNA or an RNA targeting MITF

2020-07-02 | GSE142017 | GEO

Gene expression profile of Mef2d KO retina at P14

Project description:To analyze the expression profile in the Mef2d KO retina, we performed a microarray analysis using wild-type and Mef2d KO retina at P14. We performed a microarray analysis using wild-type and Mef2d KO retina at P14, and compared the expression profiles.

2015-07-01 | E-GEOD-64861 | biostudies-arrayexpress

Expression analysis of C2C12 cells treated with scramble siRNA or siRNA directed against Brd4

Project description:We transfected C2C12 cells with 100nM of scrambled siRNA scramble and an siRNA directed against Brd4. Cells were harvested at 48h post-transfection and RNAseq was performed with polyA selection

2022-09-28 | GSE141777 | GEO

Gene expression profile of Mef2d KO retina at P14

Project description:To analyze the expression profile in the Mef2d KO retina, we performed a microarray analysis using wild-type and Mef2d KO retina at P14.

2015-07-01 | GSE64861 | GEO

MEF2D-HNRNPUL1 ChIP-seq in a genome-edited Kasumi-7 cell line

Project description:Acute lymphoblastic leukemia harboring the fusion genes involving the MEF2D transcription factor (MEF2D-ALL) is associated with poor clinical outcomes. To explore binding sites in the genome in MEF2D-ALL, we genome-edited a MEF2D-ALL cell line Kasumi-7 so that the fusion is tagged with HA at the carboxyl-terminal and co-expressed with GFP. We used this cell line for ChIP-seq using anti-HA antibody. Pair-end reads for Input and HA ChIP DNA are provided.

2020-06-30 | E-MTAB-8466 | biostudies-arrayexpress

p38γ and p38δ modulate innate immune response by regulating MEF2D activation

Project description:Evidence implicating p38γ and p38δ (p38γ/p38δ) in inflammation are mainly based on experiments using p38γ/p38δ deficient (p38γ/δ-/-) mice, which show low levels of TPL2, the kinase upstream of MKK1-ERK1/2 in myeloid cells. This could obscure p38γ/p38δ roles, since TPL2 is essential for regulating inflammation. Here we generated a p38γD171A/D171A/p38δ-/- (p38γ/δKIKO) mouse, expressing kinase-inactive p38γ and lacking p38δ. This mouse exhibited normal TPL2 levels, making it an excellent tool to elucidate specific p38γ/p38δ functions. p38γ/δKIKO mice showed a reduced inflammatory response and less susceptibility to LPS-induced septic shock and Candida albicans infection than wild-type mice. Gene expression analyses in LPS-activated WT and p38γ/δKIKO macrophages revealed that p38γ/p38δ regulated numerous genes implicated in innate immune response. Additionally, phospho-proteomic analyses and in vitro kinase assays showed that the transcription factor myocyte enhancer factor-2D (MEF2D) was phosphorylated at Ser444 via p38γ/p38δ. Mutation of MEF2D Ser444 to the non-phosphorylatable residue Ala increased its transcriptional activity and the expression of iNOS and IL-1β mRNA. These results suggest that p38γ/p38δ govern innate immune responses by regulating MEF2D phosphorylation and transcriptional activity.

2023-08-10 | PXD042626 | Pride

MEF2D binding sites in rat hippocampal neurons

Project description:We analyzed the binding profiles of MEF2D, a member of MEF2 family transcription factors, in rat hippocampal neurons. Keywords: ChIP-chip

2009-01-15 | GSE13551 | GEO

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