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Differentiating factors of cumulus cells related to quality of the human oocyte

ABSTRACT: Single embryo transfer (SET) is the most successful way to reduce the frequency of multiple pregnancies during in vitro fertilisation. However, selecting the embryo with the highest chances of pregnancy for SET remains a difficult challenge, since morphological and kinetics criteria provide poor prediction of both developmental and implantation ability. Partly through the expression of specific genes, the oocyte-cumulus interaction helps the oocyte to acquire its developmental competence. Our aim was therefore to identify at the level of cumulus cells (CCs) any genes related to oocyte developmental competence. This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE37277 | GEO | 2012/12/10

SECONDARY ACCESSION(S): PRJNA159251

REPOSITORIES: GEO

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Differentiating factors of cumulus cells related to quality of the human oocyte

Project description:Single embryo transfer (SET) is the most successful way to reduce the frequency of multiple pregnancies during in vitro fertilisation. However, selecting the embryo with the highest chances of pregnancy for SET remains a difficult challenge, since morphological and kinetics criteria provide poor prediction of both developmental and implantation ability. Partly through the expression of specific genes, the oocyte-cumulus interaction helps the oocyte to acquire its developmental competence. Our aim was therefore to identify at the level of cumulus cells (CCs) any genes related to oocyte developmental competence. This SuperSeries is composed of the SubSeries listed below. Refer to individual Series.

2012-12-10 | E-GEOD-37277 | biostudies-arrayexpress

Transcriptome based identification of mouse cumulus cell markers that predict the developmental competence of their enclosed antral oocytes

Project description:The cumulus cells (CCs) that envelope antral and ovulated oocytes have been regarded as putative source of non-invasive markers of the oocyte developmental competence and a number of studies have observed a correlation between CCs gene expression, embryo quality and final pregnancy outcome. With the aim of identifying marker transcripts, we isolated CCs from antral mouse oocytes of known developmental incompetence (NSN-CCs) or competence (SN-CCs) and compared their transcriptomes. Total RNA was isolated from cumulus cells derived from oocytes with a SN or NSN chromatin organization.

2013-09-01 | E-GEOD-46906 | biostudies-arrayexpress

Transcriptome based identification of mouse cumulus cell markers that predict the developmental competence of their enclosed antral oocytes

2013-09-01 | GSE46906 | GEO

The bovine cumulus proteome of cumulus oocyte complexes that successfully matured or failed to mature after maturation in-vitro and in-vivo

Project description:The oocyte forms a complex with their somatic cumulus cells within the follicle throughout the preovulatory maturation steps. Cumulus cells support their oocyte not only through mechanical protection but also with a close bidirectional exchange of metabolites. Analysis of the oocytes cumulus gives the opportunity to explore non-invasively oocytal well-being and quality. In vitro maturation (IVM) is the first rate-limiting step in in vitro embryo production. Analysis of protein expression in cumulus cells around this critical step helps to explore the impact of maturation conditions and to examine an influence on maturational competence of the oocyte. The goal of this study was the comparison of the cumulus proteome of oocytes with and without maturational competence matured under in vivo and in vitro conditions. Therefore twenty cumulus samples corresponding to single oocytes were analysed. Half of the samples were matured in vivo and the other half in vitro. For each maturation group, cumulus from oocytes matured successfully (SM; n=5) and failed to mature (FM; n=5) were analysed.

2020-06-30 | PXD016679 | Pride

Cumulus cell gene expression associated with pre-ovulatory acquisition of developmental competence in bovine oocytes

Project description:This study investigated the cumulus cell (CC) transcriptomic changes during the oocyte developmental competence acquisition period. Six dairy cows were used for 24 oocyte collections and received FSH twice daily over 3 days, followed by FSH withdrawal for 20, 44, 68 and 92 h in four different oestrous cycles for each of the six cows. Half of the cumulusâoocyte complexes were subjected to in vitro maturation, fertilisation and culture to assess blastocyst rate. The other half of the CC underwent microarray analysis and qRT-PCR.

2016-04-01 | E-GEOD-79760 | biostudies-arrayexpress

RNA‐sequencing reveals genes linked with oocyte developmental potential in bovine cumulus cells

Project description:Cumulus cells provide an interesting biological material to perform analyses to understand the molecular clues determining oocyte competence. The objective of this study was to analyze the transcriptional differences between cumulus cells from oocytes exhibiting different developmental potentials following individual in vitro embryo production by RNA‐seq. Cumulus cells were allocated into three groups according to the developmental potential of the oocyte following fertilization: (1) oocytes developing to blastocysts (Bl+), (2) oocytes cleaving but arresting development before the blastocyst stage (Bl-), and (3) oocytes not cleaving (Cl-).

2024-03-12 | GSE261131 | GEO

Differentiating factors of cumulus cells related to quality of the human oocyte [CadoretPart3]

Project description:Our aim was to identify at the level of cumulus cells any genes related to oocyte developmental competence 2-condition experiment: blastocyst (B) AND embryo of poor quality (E).

2012-12-10 | E-GEOD-37116 | biostudies-arrayexpress

Differentiating factors of cumulus cells related to quality of the human oocyte [CadoretPart2]

Project description:Our aim was to identify at the level of cumulus cells any genes related to oocyte developmental competence 2-condition experiment: blastocyst (B) ans embryo of poor quality (E).

2012-12-10 | E-GEOD-37117 | biostudies-arrayexpress

Differentiating factors of cumulus cells related to quality of the human oocyte [CadoretPart1]

Project description:Our aim was to identify at the level of cumulus cells any genes related to oocyte developmental competence three-condition experiment: blastocyst (B), embryo of poor quality (E) AND immature oocytes (VG).

2012-12-10 | E-GEOD-37110 | biostudies-arrayexpress

Expression data from human cumulus cells isolated from oocytes at MI and MII stages in polycystic ovary syndrome (PCOS) patients

Project description:Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder that is characterized by increased circulating androgen levels, anovulatory infertility, and frequently, insulin resistance and hyperinsulinemia.The abnormity of oocyte nuclear maturity is the main reason for anovulatory infertility and pregnancy loss in PCOS patients.The bidirectional exchanges between oocyte and contiguous CCs are important for oocyte competence acquisition, early embryonic development and CC expansion.Gene expression profiles of CCs has been suggested to predict embryo development and pregnancy outcome. We used microarrays to detail the global programme of gene expression of CCs isolated from oocytes at metaphase I (CCMI) and metaphase II (CCMII) stage under controlled ovarian stimulation (COS) cycle in PCOS patients. Cumulus cells were isolated from oocyte at stage metaphase 1(MI)and stage metaphase II (MII) of PCOS patients for RNA extraction and hybridization on Affymetrix microarrays. For microarray analysis, we used three chips for each CC category. That is, CCMI1,CCMI2,CCMI3,CCMII1,CCMII2 and CCMII3.

2013-06-05 | E-GEOD-40400 | biostudies-arrayexpress

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